The Efficacy of Autologous Myoblast Sheet Transplantation to Prevent Perforation After Duodenal Endoscopic Submucosal Dissection in Porcine Model

• Material Type: 博士論文
• ISSN: 0963-6897
• Title: The Efficacy of Autologous Myoblast Sheet Transplantation to Prevent Perforation After Duodenal Endoscopic Submucosal Dissection in Porcine Model
• Volume: 29
• Author/Editor: 松本, 亮
• Author Heading: 松本, 亮
• Publication, Distribution, etc.: SAGE Publications
• Publication Date: 2021-03-03
• Publication Date (W3CDTF): 2021-03-03
• Alternative Title: ブタ十二指腸内視鏡的粘膜下層剥離術モデルに対する自家筋芽細胞シートの穿孔抑制効果
• Periodical title: Cell Transplantation
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2021-03-03
• Date Granted (W3CDTF): 2021-03-03
• Dissertation Number: 甲医歯薬第1301号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲医歯薬第1301号
• Text Language Code: eng
• Subject Heading: duodenum; neoplasm; endoscopic resection; regenerative medicine; cell sheet technology
• Target Audience: 一般
• Note (General): The recent advent of endoscopy has enabled the endoscopic submucosal dissection (ESD) of superficial nonampullary duodenal epithelial tumors. However, the substantially thin wall and presence of bile and pancreatic juice make it technically difficult to perform duodenal ESD without perforation, which leads to lethal complications. The present study evaluated the efficacy of autologous myoblast sheet transplantation for the prevention of late perforation after duodenal ESD in a porcine model. Two weeks before ESD, skeletal muscle was surgically excised from the femur of pigs, and myoblasts were isolated and seeded in temperature-responsive culture dishes to prepare sheets. Immediately after ESD, the autologous myoblast sheets were attached to the serosal surface at the ESD site with omentopexy. The pigs were divided into two groups: the autologous myoblast sheet group (n ¼ 5), where the myoblast cell sheet was attached to the ESD ulcer part from the duodenal serous side, and the Omentum group (n ¼ 5), where only the omentum was used. The pigs were sacrificed and analyzed macroscopically and histologically on postoperative day 3. The macroscopic examination of the abdominal cavity revealed perforation in the ESD ulcer area and leakage of bile in the Omentum group but no perforation in the Sheet group. A histopathological examination revealed that continuity of the duodenal wall at the ESD site was maintained with dense connective tissue in the Sheet group. In conclusion, autologous myoblast sheets were useful for preventing perforation after duodenal ESD.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1301号 学位授与年月日:令和3年3月3日; Author: Ryo Matsumoto, Kengo Kanetaka, Yasuhiro Maruya, Shun Yamaguchi, Shinichiro Kobayashi, Daisuke Miyamoto, Ken Ohnita, Yusuke Sakai, Keiichi Hashiguchi, Kazuhiko Nakao and Susumu Eguchi; Citation: Cell Transplantation, 29(1–12),pp.1-12 ; 2020; identifier:Nagasaki University (長崎大学), 博士(医学) (2021-03-03); http://hdl.handle.net/10069/40570
• DOI: info:doi/10.1177/0963689720963882; https://doi.org/info:doi/10.1177/0963689720963882
• Persistent ID (NDL): info:ndljp/pid/11671554; https://dl.ndl.go.jp/pid/11671554
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2021-05-16T23:51:55+09:00
• Date Created (W3CDTF): 2021-04-22
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Related Material (URI): http://hdl.handle.net/10069/40570
• Periodical Title (URI): http://hdl.handle.net/10069/00040737
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp