Anisomycin, a JNK and p38 activator, suppresses cell-cell junction formation in 2D cultures of K38 mouse keratinocyte cells and reduces claudin-7 expression, with an increase of paracellular permeability in 3D cultures.

• Material Type: 博士論文
• Title: Anisomycin, a JNK and p38 activator, suppresses cell-cell junction formation in 2D cultures of K38 mouse keratinocyte cells and reduces claudin-7 expression, with an increase of paracellular permeability in 3D cultures.
• Author/Editor: Nikaido, Misaki; Otani, Takahito; Kitagawa, Norio; Ogata, Kayoko; Iida, Hiroshi; Anan, Hisashi; Inai, Tetsuichiro
• Author Heading: Nikaido, Misaki; Otani, Takahito; Kitagawa, Norio; Ogata, Kayoko; Iida, Hiroshi; Anan, Hisashi; Inai, Tetsuichiro
• Publication Date: 2019-03-02
• Publication Date (W3CDTF): 2019-03-02
• Alternative Title: Anisomycin, a JNK and p38 activator, suppresses cell-cell junction formation in 2D cultures of K38 mouse keratinocyte cells and reduces claudin-7 expression, with an increase of paracellular permeability in 3D cultures.
• Degree grantor/type: 福岡歯科大学
• Date Granted: 2019-03-02
• Date Granted (W3CDTF): 2019-03-02
• Dissertation Number: 甲第305号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第305号
• Text Language Code: eng
• Subject Heading: c-Jun NH2-terminal protein kinase (JNK); p38 mitogen-activated protein kinase (MAPK); tight junction; claudin; keratinocyte; three-dimensional culture
• Target Audience: 一般
• Note (General): Keratinocytes in the oral mucosal epithelium, which is a non-keratinized stratified epithelium, are exposed to various stimuli from the oral cavity. JNK and p38 are stress-activated mitogen-activated protein kinases (MAPKs) that are phosphorylated by various stimuli and are involved in the assembly and disassembly of tight junctions (TJs) in keratinocytes. Therefore, we investigated the effects of stress-activated MAPKs on TJs in a mouse keratinocyte cell line during cell-cell junction formation in two-dimensional (2D) cultures or stratification to form non-keratinized epithelium in 3D cultures. In 2D cultures, calcium induced zipper-like staining for ZO-1 at 2h and string-like staining for ZO-1 at 12h, which indicated immature and mature cell-cell junctions, respectively. Anisomycin (AM), a JNK and p38 activator, inhibited formation of string-like staining for ZO-1, whereas inhibition of JNK, but not p38, after AM treatment restored string-like staining for ZO-1, although claudins (CLDNs) 4, 6, and 7 did not completely colocalize to ZO-1-positive sites. In 3D cultures, AM treatment for 2 weeks activated only p38, suppressed flattening of the superficial cells, removed CLDN7 from ZO-1-positive spots on the surface of 3D cultures, which represent TJs, and decreased transepithelial electrical resistance. Thus, short-term AM treatment inhibited maturation of cell-cell junctions by JNK, but not p38, activation. p38 activation by long-term AM treatment affected morphology of stratified structures and paracellular permeability, which was increased by CLDN7 removal from TJs. Various chronic stimuli that activate stress-activated MAPKs may weaken the keratinocyte barrier and be involved in TJ-related diseases.; SpringerLink
• Persistent ID (NDL): info:ndljp/pid/11673174; https://dl.ndl.go.jp/pid/11673174
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2021-05-20T03:24:30+09:00
• Date Created (W3CDTF): 2019-04-11
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Original Material (URI): https://doi.org/10.1007/s00418-018-1736-z
• Periodical Title (URI): https://fdc.repo.nii.ac.jp/records/103
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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