Author/EditorGhavidel Darestani, Sahar
Alternative TitleAssociation between changes in the mRNA expression of platelet-activating factor receptor in peripheral blood mononuclear cells and progression of diabetic nephropathy
Periodical titleDiabetology International
Degree Type博士(医学)
Doctor of Philosophy in Medical Science
Note (General)博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨
Introduction: Several studies have recently pointed out the role of many inflammatory mediators in the progression of diabetes complications. We had previously demonstrated that mRNA expression of platelet activating factor receptor (PAFR) in peripheral blood mononuclear cells (PBMCs) was associated with urinary albumin to creatinine ratio (ACR) and forearm flow-mediated dilatation in patients with type 2 diabetes. In an attempt to elucidate this association, patients were followed up for 1 year.Materials and Methods: We recruited 95 patients from the hospital outpatient clinic, among whom 86 were followed up for 1 year (normoalbuminuria: 40 patients, microalbuminuria: 25 patients, macroalbuminuria: 21 patients). We then measured their baseline and 12 month characteristics and collected blood samples to extract PBMCs and measure gene expressions.Results: Despite higher mRNA expression of PAFR in PBMCs among patients with macroalbuminuria, the rise in its value was not associated with biomarkers of nephropathy, while baseline values were not associated with progression of nephropathy. Moreover, changes in mRNA expression of PAFR were correlated with changes in ACR in all patients (r = 0.225, p = 0.037) and estimated glomerular filtration rate in patients with macroalbuminuria (r = −0.438, p = 0.047) during the follow-up period.Conclusion: Our findings indicate that even though no causal relationship exists between diabetic nephropathy and elevated expression of PAFR in PBMCs, their close association; signifies the presence of another common mechanism that could induce both events. Given these findings, the PAF/PAFR interaction could clarify corresponding mechanisms involved in diabetic complications.Sahar Ghavidel Darestani, Mihoko Kurano, Atsushi Shinnakasu, Kiyoaki Yamamoto, Yukari Dochi, Kayo Uemura, Yuko Ikeda, Aiko Arimura, Hiroshi Arimura, Akira Kikuchi, Hiroshi Hashiguchi, Takahisa Deguchi, Yoshihiko NishioAssociation between changes in the mRNA expression of platelet activating factor receptor in peripheral blood mononuclear cells and progression of diabetic nephropathyDiabetology International, 11, 11–18(2020)This is a post-peer-review, pre-copyedit version of an article published in Diabetology International.The final authenticated version is available online at:https://doi.org/10.1007/s13340-019-00394-w
Collection (particular)国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
Date Accepted (W3CDTF)2021-05-20T03:24:30+09:00
Data Provider (Database)国立国会図書館 : 国立国会図書館デジタルコレクション