Studies on toxicological effects of lead in animals for evaluation of worldwide environmental lead pollution

• Material Type: 博士論文
• Title: Studies on toxicological effects of lead in animals for evaluation of worldwide environmental lead pollution
• Author/Editor: Kataba, Andrew
• Author Heading: Kataba, Andrew
• Publication, Distribution, etc.: Hokkaido University
• Publication Date: 2021-03-25
• Publication Date (W3CDTF): 2021-03-25
• Alternative Title: 環境中の鉛汚染評価のための生物における鉛の毒性影響に関する研究
• Contributor: 坪田, 敏男; 石塚, 真由美; 齋藤, 健; 中山, 翔太
• Degree grantor/type: 北海道大学
• Date Granted: 2021-03-25
• Date Granted (W3CDTF): 2021-03-25
• Dissertation Number: 甲第14541号
• Degree Type: 博士(獣医学)
• Conferring No. (Dissertation): 甲第14541号
• Text Language Code: eng
• Subject Heading: fish; lead; heart rate; gene expression; apoptosis; spasm
• NDC: 649
• Target Audience: 一般
• Note (General): Ubiquitous environmental lead (Pb) contamination is related to human activities with mining as one of the major contributors. A long history of lead-zinc mining in Kabwe, Zambia extensively polluted the environment and some isolated water bodies with lead. To this end, I explored the negative effects of the current Kabwe lead water levels on aquatic life using 2.5 hours post fertilization (hpf) old embryos zebrafish (Danio rerio) exposed to a wide range of Pb concentrations (1-5000 μg/L Pb) until 96 hpf. Exposure negatively impacted the development and survival of zebrafish embryos by inducing embryo coagulation mortalities, reduced hatching rates, lack of embryo hatching in a concentration dependant manner. At 24 hpf, elevated embryo burst activity at lowest level of exposure (1 μg/L Pb) and decreased burst count per minute beginning at 500 μg/L were recorded. At 100 – 500 μg/L Pb, exposure induced cardiovascular toxicity by reducing heart rate and increasing blood flow activity concurrently. At 72 hpf involuntary muscular twitching that increased in number and duration were recorded at 50-500 μg/L Pb. The Pb toxicity dysregulation of the oxidative stress system evidenced by downregulation of catalase, and upregulation of heme oxyeganse-1, glutathione-S-transferase, cytochrome c oxidase subunit I mRNA gene expressions. The proapoptotic gene, tumour protein 53 expression level were downregulated across exposed groups. In contrast, the antiapoptotic gene, B-cell lymphoma 2 were upregulated at the lowest exposure spectrum (1-25 μg/L) and downregulated from 50-500 μg/L Pb. The findings suggest that the developmental Pb exposure in zebrafish may be accompanied with deleterious effects within environmentally and regulatory Pb levels.; (主査) 教授 坪田 敏男, 教授 石塚 真由美, 教授 齋藤 健（保健科学研究院）, 助教 中山 翔太; 獣医学院（獣医学専攻）
• DOI: 10.14943/doctoral.k14541; https://doi.org/10.14943/doctoral.k14541
• Persistent ID (NDL): info:ndljp/pid/11690898; https://dl.ndl.go.jp/pid/11690898
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2021-07-05T22:24:43+09:00
• Date Created (W3CDTF): 2021-03
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://dx.doi.org/10.14943/doctoral.k14541; http://hdl.handle.net/2115/81709
• Is Referenced By (URI): http://hdl.handle.net/2115/81624
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

• Summary, etc.: Ubiquitous environmental lead (Pb) contamination is related to human activities with mining as one of the major contributors. A long history of lead-z...