The immunoregulatory role of p21 in the development of the temporomandibular joint- osteoarthritis

• Material Type: 博士論文
• ISSN: 2057-4347
• Title: The immunoregulatory role of p21 in the development of the temporomandibular joint- osteoarthritis
• Volume: 7 3
• Author/Editor: khurel-Ochir, Tsendsuren; Izawa, Takashi; Iwasa, Akihiko; Kano, Fumiya; Yamamoto, Akihito; Tanaka, Eiji
• Author Heading: khurel-Ochir, Tsendsuren; Izawa, Takashi; Iwasa, Akihiko; Kano, Fumiya; Yamamoto, Akihito; Tanaka, Eiji
• Publication, Distribution, etc.: Wiley
• Publication Date: 2021-02-10
• Publication Date (W3CDTF): 2021-02-10
• Alternative Title: 変形性顎関節症の進展におけるp21の免疫調節作用
• Periodical title: Clinical and Experimental Dental Research
• Degree grantor/type: 徳島大学
• Date Granted: 2021-03-23
• Date Granted (W3CDTF): 2021-03-23
• Dissertation Number: 甲第3493号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第3493号
• Text Language Code: eng
• Subject Heading: p21; chondrocytes; temporomandibular joint; osteoarthritis; metalloprotease; chondrocyte
• Target Audience: 一般
• Note (General): Objective: We aimed to identify the immunoregulatory role of the cyclin-dependent kinase inhibitor p21 in the homeostasis of mandibular condylar cartilage affected by mechanical stress.Materials and Methods: Ten C57BL/6 wild-type (WT) and ten p21–/– mice aged 8 weeks were divided into the untreated and treated groups. In the treated groups, mechanical stress was applied to the temporomandibular joint (TMJ) through forced mouth opening for 3 h/day for 7 days. The dissected TMJs were assessed using micro-CT, histology, and immunohistochemistry.Results: Treated p21–/– condyles with mechanical stress revealed more severe subchondral bone destruction, with thinner cartilage layers and smaller proteoglycan area relative to treated WT condyles; untreated WT and p21–/– condyles had smoother surfaces. Immunohistochemistry revealed significant increases in the numbers of Caspase-3, interleukin-1β, matrix metalloprotease (MMP)-9, and MMP-13 positive cells, and few aggrecan positive cells, in treated p21–/– than in treated WT samples. Moreover, the number of TUNEL positive cells markedly increased in p21–/– condyles affected by mechanical stress.Conclusion: Our findings indicate that p21 in chondrocytes contributes to regulate matrix synthesis via the control of aggrecan and MMP-13 expression under mechanical stress. Thus, p21 might regulate the pathogenesis of osteoarthritis in the TMJ.
• DOI: info:doi/10.1002/cre2.404; https://doi.org/info:doi/10.1002/cre2.404
• Persistent ID (NDL): info:ndljp/pid/11691206; https://dl.ndl.go.jp/pid/11691206
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2021-07-05T22:24:43+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/116060
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp