Quantitative visualization of stable isotope-labeled chromosome using isotope nanoscope

• Material Type: 博士論文
• Title: Quantitative visualization of stable isotope-labeled chromosome using isotope nanoscope
• Author/Editor: 永田, 康祐
• Author Heading: 永田, 康祐
• Publication, Distribution, etc.: Hokkaido University
• Publication Date: 2021-03-25
• Publication Date (W3CDTF): 2021-03-25
• Alternative Title: 同位体ナノスコープによる安定同位体標識された染色体の定量的な可視化
• Contributor: 圦本, 尚義; 中川, 光弘; 川野, 潤; 馬上, 謙一
• Degree grantor/type: 北海道大学
• Date Granted: 2021-03-25
• Date Granted (W3CDTF): 2021-03-25
• Dissertation Number: 甲第14366号
• Degree Type: 博士(理学)
• Conferring No. (Dissertation): 甲第14366号
• Text Language Code: eng
• Alias of Author: Nagata, Kosuke
• NDC: 400
• Target Audience: 一般
• Note (General): Visualization of DNA strands and chromosomes have been used for the studies of DNA replication, resistance for mutagenesis and DNA repairing, and developed by using radio isotopes or analogs to label nucleotides throughout the cell cycles. However, these labels incorporated into DNA strand restrict the analysis of long-term dynamics of chromosomes because of its mutagenesis resulting in the mutations in cells. We applied stable isotopes for the nucleotide labeling to analyze spontaneous functions of chromosome. In this study, the imaging performance of isotope nanoscope and the consistence of the changes of the label amounts in the chromosomes were studied to evaluate the traceability of stable isotope labels. Firstly, the diameters of primary ion beam with aberration correction mode and non-correction mode were compared with the primary ion current to assess the imaging performance of isotope nanoscope. Aberration corrected primary ion beam showed smaller diameter and twice higher current density at a given lateral resolution. As a result, isotope nanoscope with aberration corrected primary ion beam indicated the abilities to produce fine and dense primary ion beam with lower acceleration voltage resulting in higher lateral resolutions and sensitivities and lower sample disruptions compared with conventional imaging mass spectrometers. Then, the chromosomes in human cultured cell labeled by U-13C6-Glucose throughout the cell cycles were visualized by isotope nanoscope. The heterogeneities of carbon isotope abundance were observed in each chromosome, and they reflected the semi-conservative replication of DNA strands. The distributions of stable isotope labels also suggested dispersive histone-incorporations into the chromatids. This nonmutagenic and subcellular resolution imaging may be useful to analyze natural functions of organelles with in single cells without radio isotope or analog usages.; (主査) 教授 圦本 尚義, 教授 中川 光弘, 准教授 川野 潤, 助教 馬上 謙一; 理学院（自然史科学専攻）
• DOI: 10.14943/doctoral.k14366; https://doi.org/10.14943/doctoral.k14366
• Persistent ID (NDL): info:ndljp/pid/11692169; https://dl.ndl.go.jp/pid/11692169
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2021-07-05T22:24:43+09:00
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://dx.doi.org/10.14943/doctoral.k14366; http://hdl.handle.net/2115/81966
• Is Referenced By (URI): http://hdl.handle.net/2115/81965
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

• Summary, etc.: Visualization of DNA strands and chromosomes have been used for the studies of DNA replication, resistance for mutagenesis and DNA repairing, and de...