Suppressive effects of quercetin on hydrogen peroxide-induced caveolin-1 phosphorylation in endothelial cells

• Material Type: 博士論文
• ISSN: 0912-0009
• Title: Suppressive effects of quercetin on hydrogen peroxide-induced caveolin-1 phosphorylation in endothelial cells
• Volume: 69 1
• Author/Editor: Kondo-Kawai, Akari; Sakai, Tohru; Terao, Junji; Mukai, Rie
• Author Heading: Kondo-Kawai, Akari; Sakai, Tohru; Terao, Junji; Mukai, Rie
• Publication, Distribution, etc.: Society for Free Radical Research Japan
• Publication Date: 2021-07-01
• Publication Date (W3CDTF): 2021-07-01
• Alternative Title: 血管内皮細胞における過酸化水素誘導のカベオリン-1リン酸化に対するケルセチンの抑制効果
• Periodical title: Journal of Clinical Biochemistry and Nutrition
• Degree grantor/type: 徳島大学
• Date Granted: 2021-03-23
• Date Granted (W3CDTF): 2021-03-23
• Dissertation Number: 甲第3510号
• Degree Type: 博士（栄養学）
• Conferring No. (Dissertation): 甲第3510号
• Text Language Code: eng
• Subject Heading: quercetin; caveolae; caveolin-1; hydrogen peroxide; vascular endothelium
• Target Audience: 一般
• Note (General): Caveolin-1 is a major protein of the caveolae structure in vascular endothelial cell membrane. Phosphorylation of caveolin-1 is one of the initial events leading to exacerbation of vascular permeability caused by oxidative stress. Although quercetin is known to be an anti-atherosclerosis factor that acts as a dietary antioxidant, little is known about its role in the regulation of caveolin-1 phosphorylation. In this study, we investigated the inhibitory effect of quercetin on hydrogen peroxide-induced caveolin-1 phosphorylation in human umbilical vein endothelial cells. Quercetin inhibited caveolin-1 phosphorylation in cells pretreated with quercetin for 24 h and then exposed to hydrogen peroxide. However, quercetin 3-O-β-glucuronide, a conjugated metabolite of quercetin, did not exert this inhibitory effect. Exposure to hydrogen peroxide increased vascular permeability and reduced mRNA expression of the intercellular adhesion protein, vascular endothelial cadherin (VE-cadherin). By contrast, pretreatment with quercetin suppressed the increase in vascular permeability and decreased VE-cadherin expression. These results indicate that deconjugated quercetin can play a role in the prevention of altered vascular permeability under oxidative stress by suppressing caveolin-1 phosphorylation. Thus, dietary quercetin may be beneficial for the maintenance of endothelial cell function.
• DOI: info:doi/10.3164/jcbn.20-190; https://doi.org/info:doi/10.3164/jcbn.20-190
• Persistent ID (NDL): info:ndljp/pid/11710969; https://dl.ndl.go.jp/pid/11710969
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2021-08-09T17:15:52+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/116063
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp