The Differences in the Characteristics of Insulin-producing Cells Using Human Adipose-tissue Derived Mesenchymal Stem Cells from Subcutaneous and Visceral Tissues

• Material Type: 博士論文
• ISSN: 2045-2322
• Title: The Differences in the Characteristics of Insulin-producing Cells Using Human Adipose-tissue Derived Mesenchymal Stem Cells from Subcutaneous and Visceral Tissues
• Volume: 9
• Author/Editor: Wada, Yuma; Ikemoto, Tetsuya; Morine, Yuji; Imura, Satoru; Saito, Yu; Yamada, Shinichiro; Shimada, Mitsuo
• Author Heading: Wada, Yuma; Ikemoto, Tetsuya; Morine, Yuji; Imura, Satoru; Saito, Yu; Yamada, Shinichiro; Shimada, Mitsuo
• Publication, Distribution, etc.: Springer Nature
• Publication Date: 2019-09-13
• Publication Date (W3CDTF): 2019-09-13
• Alternative Title: 脂肪由来間葉系幹細胞からインスリン産生細胞への分化誘導に際しての皮下および腹腔内脂肪の特性の差異に関する研究
• Periodical title: Scientific Reports
• Degree grantor/type: 徳島大学
• Date Granted: 2021-06-24
• Date Granted (W3CDTF): 2021-06-24
• Dissertation Number: 甲第3530号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第3530号
• Text Language Code: eng
• Subject Heading: ADSC; IPC; subcutaneous fat tissue; visceral fat tissue
• Target Audience: 一般
• Note (General): The aim of this study was to investigate the characteristics of insulin producing cells (IPCs) differentiated from adipose-tissue derived stem cells (ADSCs) isolated from human subcutaneous and visceral adipose tissues and identify ADSCs suitable for differentiation into efficient and functional IPCs. Subcutaneous and visceral adipose tissues collected from four (4) patients who underwent digestive surgeries at The Tokushima University (000035546) were included in this study. The insulin secretion of the generated IPCs was investigated using surface markers by: fluorescence activated cell sorting (FACS) analysis; cytokine release; proliferation ability of ADSCs; in vitro (glucose-stimulated insulin secretion: (GSIS) test/in vivo (transplantation into streptozotocin-induced diabetic nude mice). The less fat-related inflammatory cytokines secretions were observed (P < 0.05), and the proliferation ability was higher in the subcutaneous ADSCs (P < 0.05). Insulin expression and GISI were higher in the subcutaneous IPCs (P < 0.01 and P < 0.05, respectively). The hyperglycaemic state of all mice that received IPCs from subcutaneous fat tissue converted into normo-glycaemia in thirty (30) days post-transplantation (4/4,100%). Transplanted IPCs were stained using anti-insulin and anti-human leukocyte antigen antibodies. The IPCs generated from the ADSCs freshly isolated from the human fat tissue had sufficient insulin secreting ability in vitro and in vivo.
• DOI: info:doi/10.1038/s41598-019-49701-0; https://doi.org/info:doi/10.1038/s41598-019-49701-0
• Persistent ID (NDL): info:ndljp/pid/11710970; https://dl.ndl.go.jp/pid/11710970
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2021-08-09T17:15:52+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/114942
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp