EphA7+perivascular cells as myogenic and angiogenic precursors improving skeletal muscle regeneration in a muscular dystrophic mouse model. （EphA7陽性周細胞は、筋ジストロフィーマウスモデルにおいて、骨格筋再生能を改善する）

• Material Type: 博士論文
• ISSN: 1873-5061
• Title: EphA7+perivascular cells as myogenic and angiogenic precursors improving skeletal muscle regeneration in a muscular dystrophic mouse model. （EphA7陽性周細胞は、筋ジストロフィーマウスモデルにおいて、骨格筋再生能を改善する）
• Volume: 47
• Author/Editor: 鹿野, 耕平
• Publication Date: 2020-07
• Publication Date (W3CDTF): 2020-07
• Periodical title: Stem cell research
• Degree grantor/type: 旭川医科大学
• Date Granted: 2020-09-30
• Date Granted (W3CDTF): 2020-09-30
• Dissertation Number: 甲第549号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第549号
• Text Language Code: eng
• Subject Heading: Capillary; Muscular dystrophy; Myogenesis; Pericytes; Skeletal muscle; Stem cells
• Note (General): Skeletal muscle has a capacity for muscular regeneration mediated by satellite cells (SCs) and non-SCs. Although it is proposed that non-SCs are attractive therapeutic targets for dystrophies, the biological properties of these cells remain unclear. We have recently identified novel multipotent pericytes (PCs), capillary stem cells (CapSCs) derived from the microvasculature. In the present study, we determined if CapSCs contributed to myogenic regeneration using muscular dystrophy mouse model. CapSCs were isolated as EphA7+NG2+PCs from the subcutaneous adipose tissues of GFP-transgenic mice. Co-culture with C2C12 myoblast cells showed that CapSCs effectively enhanced myogenesis as compared to controls including EphA7- PCs and adipose stromal cells (ASCs). CapSCs transplanted in cardiotoxin-injured gastrocnemius muscles were well differentiated into both muscle fibers and microvessels, as compared to controls. At three weeks after cell-transplantation into the limbs of the mdx/utrn-/-mouse, CapSCs increased the number of GFP+myofibers along with dystrophin expression and the area size of myofibers, and also enhanced the muscular mass and its performance, assessed by treadmill test as compared to controls. In conclusion, CapSCs have potent myogenic regeneration capacity and improved the pathological condition in a muscular dystrophy mouse. Thus, CapSCs are an attractive cellular source in regenerative therapy for muscular dystrophy.; identifier:PMID: 32738632; 開始ページ : 101914
• DOI: info:doi/10.1016/j.scr.2020.101914; https://doi.org/info:doi/10.1016/j.scr.2020.101914
• Persistent ID (NDL): info:ndljp/pid/11765570; https://dl.ndl.go.jp/pid/11765570
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2021-10-05T14:27:03+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://amcor.asahikawa-med.ac.jp/modules/xoonips/detail.php?id=20200930_K549
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp