Morin attenuates dexamethasone-mediated oxidative stress and atrophy in mouse C2C12 skeletal myotubes

• Material Type: 博士論文
• ISSN: 0003-9861
• Title: Morin attenuates dexamethasone-mediated oxidative stress and atrophy in mouse C2C12 skeletal myotubes
• Volume: 704
• Author/Editor: Anaytulla,; Uchida, Takayuki; Miki, Yukari; Sugiura, Kosuke; Higashitani, Atsushi; Kobayashi, Takeshi; Ohno, Ayako; Nakao, Reiko; Hirasaka, Katsuya; Sakakibara, Iori; Nikawa, Takeshi
• Author Heading: Anaytulla,; Uchida, Takayuki; Miki, Yukari; Sugiura, Kosuke; Higashitani, Atsushi; Kobayashi, Takeshi; Ohno, Ayako; Nakao, Reiko; Hirasaka, Katsuya; Sakakibara, Iori; Nikawa, Takeshi
• Publication, Distribution, etc.: Elsevier
• Publication Date: 2021-04-10
• Publication Date (W3CDTF): 2021-04-10
• Alternative Title: C2C12筋管細胞においてモリンはデキサメタゾン誘導性の酸化ストレスと筋萎縮を抑制する
• Periodical title: Archives of Biochemistry and Biophysics
• Degree grantor/type: 徳島大学
• Date Granted: 2021-09-02
• Date Granted (W3CDTF): 2021-09-02
• Dissertation Number: 甲第3532号
• Degree Type: 博士（栄養学）
• Conferring No. (Dissertation): 甲第3532号
• Text Language Code: eng
• Subject Heading: Muscle atrophy; Morin; Mouse C2C12 skeletal myotubes; Reactive oxygen species; Ubiquitin ligases
• Target Audience: 一般
• Note (General): Glucocorticoids are the drugs most commonly used to manage inflammatory diseases. However, they are prone to inducing muscle atrophy by increasing muscle proteolysis and decreasing protein synthesis. Various studies have demonstrated that antioxidants can mitigate glucocorticoid-induced skeletal muscle atrophy. Here, we investigated the effect of a potent antioxidative natural flavonoid, morin, on the muscle atrophy and oxidative stress induced by dexamethasone (Dex) using mouse C2C12 skeletal myotubes. Dex (10 μM) enhanced the production of reactive oxygen species (ROS) in C2C12 myotubes via glucocorticoid receptor. Moreover, Dex administration reduced the diameter and expression levels of the myosin heavy chain protein in C2C12 myotubes, together with the upregulation of muscle atrophy-associated ubiquitin ligases, such as muscle atrophy F-box protein 1/atrogin-1, muscle ring finger protein-1, and casitas B-lineage lymphoma proto-oncogene-b. Dex also significantly decreased phosphorylated Foxo3a and increased total Foxo3a expression. Interestingly, Dex-induced ROS accumulation and Foxo3a expression were inhibited by morin (10 μM) pretreatment. Morin also prevented the Dex-induced reduction of myotube thickness, together with muscle protein degradation and suppression of the upregulation of atrophy-associated ubiquitin ligases. In conclusion, our results suggest that morin effectively prevents glucocorticoid-induced muscle atrophy by reducing oxidative stress.
• DOI: info:doi/10.1016/j.abb.2021.108873; https://doi.org/info:doi/10.1016/j.abb.2021.108873
• Persistent ID (NDL): info:ndljp/pid/11864503; https://dl.ndl.go.jp/pid/11864503
• Collection: 障害者向け資料
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• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2021-11-08T14:10:24+09:00
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
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• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/116344
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp