Anti-malarial activity of traditional Kampo medicine Coptis rhizome extract and its major active compounds 19

The cover of this title could differ from library to library.

Available in National Diet Library

Find on the publisher's website

国立国会図書館デジタルコレクション

Digital data available

Check on the publisher's website

DOI[info:doi/10.1186/s12936-020-03273-x]to the data of the same series

Anti-malarial activity of traditional Kampo medicine Coptis rhizome extract and its major active compounds

Persistent ID (NDL): info:ndljp/pid/11864525

Material type: 博士論文

Author: Teklemichael, Awet Alem

Publisher: BioMed Central Ltd

Publication date: 2020-06-08

Material Format: Digital

Capacity, size, etc.: -

Name of awarding university/degree: Nagasaki University (長崎大学),博士(医学)

View All

Notes on use at the National Diet Library

本資料は、掲載誌(URI)等のリンク先にある学位授与機関のWebサイトやCiNii Dissertationsから、本文を自由に閲覧できる場合があります。

Notes on use

Note (General)：: BACKGROUND: Herbal medicine has been a rich source of new drugs exemplified by quinine and artemisinin. In this study, a variety of Japanese tradition...

Search by Bookstore

Read this material in an accessible format.

View materials in accessible formats for people with print disabilities (1Type)

Search by Bookstore

Read in Disability Resources

Other Services
- テキストデータ check it on 国立国会図書館デジタルコレクション

Bibliographic Record

You can check the details of this material, its authority (keywords that refer to materials on the same subject, author's name, etc.), etc.

Digital

Material Type: 博士論文
ISSN: 1475-2875
Title: Anti-malarial activity of traditional Kampo medicine Coptis rhizome extract and its major active compounds
Volume: 19
Author/Editor: Teklemichael, Awet Alem
Author Heading: Teklemichael, Awet Alem
Publication, Distribution, etc.: BioMed Central Ltd
Publication Date: 2020-06-08
Publication Date (W3CDTF): 2020-06-08
Alternative Title: 漢方生薬オウレンとその主要活性化合物の抗マラリア活性
Periodical title: Malaria Journal
Degree grantor/type: Nagasaki University (長崎大学)
Date Granted: 2021-09-17
Date Granted (W3CDTF): 2021-09-17
Dissertation Number: 甲医歯薬第1358号
Degree Type: 博士(医学)
Conferring No. (Dissertation): 甲医歯薬第1358号
Text Language Code: eng
Subject Heading: Herbal medicine
Kampo
Antimalarial
Target Audience: 一般
Note (General): BACKGROUND: Herbal medicine has been a rich source of new drugs exemplified by quinine and artemisinin. In this study, a variety of Japanese traditional herbal medicine ('Kampo') were examined for their potential anti-malarial activities. METHODS: A comprehensive screening methods were designed to identify novel anti-malarial drugs from a library of Kampo herbal extracts (n?=?120) and related compounds (n?=?96). The anti-malarial activity was initially evaluated in vitro against chloroquine/mefloquine-sensitive (3D7) and-resistant (Dd2) strains of Plasmodium falciparum. The cytotoxicity was also evaluated using primary adult mouse brain cells. After being selected through the first in vitro assay, positive extracts and compounds were examined for possible in vivo anti-malarial activity. RESULTS: Out of 120 herbal extracts, Coptis rhizome showed the highest anti-malarial activity (IC50 1.9 μg/mL of 3D7 and 4.85 μg/mL of Dd2) with a high selectivity index (SI)?>?263 (3D7) and?>?103 (Dd2). Three major chlorinated compounds (coptisine, berberine, and palmatine) related to Coptis rhizome also showed anti-malarial activities with IC50 1.1, 2.6, and 6.0 μM (against 3D7) and 3.1, 6.3, and 11.8 μM (against Dd2), respectively. Among them, coptisine chloride exhibited the highest anti-malarial activity (IC50 1.1 μM against 3D7 and 3.1 μM against Dd2) with SI of 37.8 and 13.2, respectively. Finally, the herbal extract of Coptis rhizome and its major active compound coptisine chloride exhibited significant anti-malarial activity in mice infected with Plasmodium yoelii 17X strain with respect to its activity on parasite suppression consistently from day 3 to day 7 post-challenge. The effect ranged from 50.38 to 72.13% (P?<?0.05) for Coptis rhizome and from 81 to 89% (P?<?0.01) for coptisine chloride. CONCLUSION: Coptis rhizome and its major active compound coptisine chloride showed promising anti-malarial activity against chloroquine-sensitive (3D7) and -resistant (Dd2) strains in vitro as well as in vivo mouse malaria model. Thus, Kampo herbal medicine is a potential natural resource for novel anti-malarial agents.
長崎大学学位論文学位記番号:博(医歯薬)甲第1358号学位授与年月日:令和3年9月17日
Author: Awet Alem Teklemichael, Shusaku Mizukami, Kazufumi Toume, Farhana Mosaddeque, Mohamed Gomaa Kamel, Osamu Kaneko, Katsuko Komatsu, Juntra Karbwang, Nguyen Tien Huy & Kenji Hirayama
Citation: Malaria Journal, 19, art. no. 204; 2020
identifier:Nagasaki University (長崎大学), 博士(医学) (2021-09-17)
http://hdl.handle.net/10069/00040841
DOI: info:doi/10.1186/s12936-020-03273-x
https://doi.org/info:doi/10.1186/s12936-020-03273-x
Persistent ID (NDL): info:ndljp/pid/11864525
https://dl.ndl.go.jp/pid/11864525
Collection: 障害者向け資料
Collection (Materials For Handicapped People:1): テキストデータ
Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
https://dl.ndl.go.jp/collections/A00014
Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2021-11-08T14:10:24+09:00
Date Created (W3CDTF): 2021-10-06
Format (IMT): PDF
Access Restrictions: 国立国会図書館内限定公開
Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
Availability of remote photoduplication service: 可
Related Material (URI): http://hdl.handle.net/10069/00040841
Periodical Title (URI): http://hdl.handle.net/10069/00040926
Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション
https://dl.ndl.go.jp