Protective effects of resveratrol against 5-fluorouracil-induced oxidative stress and inflammatory responses in human keratinocytes

• Material Type: 博士論文
• ISSN: 0912-0009
• Title: Protective effects of resveratrol against 5-fluorouracil-induced oxidative stress and inflammatory responses in human keratinocytes
• Volume: 69 3
• Author/Editor: Chen, Shu; Tamaki, Naofumi; Kudo, Yasusei; Tsunematsu, Takaaki; Miki, Kaname; Ishimaru, Naozumi; Ito, Hiro-O
• Author Heading: Chen, Shu; Tamaki, Naofumi; Kudo, Yasusei; Tsunematsu, Takaaki; Miki, Kaname; Ishimaru, Naozumi; Ito, Hiro-O
• Publication, Distribution, etc.: Society for Free Radical Research Japan
• Publication Date: 2021-11-01
• Publication Date (W3CDTF): 2021-11-01
• Alternative Title: ヒト角化上皮細胞における5-FU誘発性酸化ストレスおよび炎症応答に対するresveratrolの保護効果
• Periodical title: Journal of Clinical Biochemistry and Nutrition
• Degree grantor/type: 徳島大学
• Date Granted: 2021-09-09
• Date Granted (W3CDTF): 2021-09-09
• Dissertation Number: 甲第3541号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第3541号
• Text Language Code: eng
• Subject Heading: oxidative stress; inflammation; oral mucositis; antioxidant; human normal keratinocyte; resveratrol
• Target Audience: 一般
• Note (General): Although 5-fluorouracil (5-FU) is currently used as an anti-cancer chemotherapy, adverse effects such as oral mucositis potentially limit its clinical application. Additionally, the prevention of 5-FU-induced side effects are scarce. Resveratrol is known to decrease oxidative damage and inflammation. In this study, we examined the protective effects of resveratrol on 5-FU-induced oxidative stress and inflammatory responses in normal human keratinocytes (HaCaT cell) as in vitro oral mucositis model. HaCaT cells were exposed to 5-FU and simultaneously treated with resveratrol. The effects of resveratrol on 5-FU-induced cytotoxicity were evaluated using cell viability assay. The production of reactive oxygen species (ROS) was measured using a fluorescence spectrophotometer. The effects of resveratrol on nuclear factor erythroid 2-related factor 2 (Nrf2), silent information regulator transcript-1 (SIRT-1), and nuclear factor kappa B (NF-κB) signaling and inflammatory cytokine expression were examined. Resveratrol suppressed 5-FU-induced overproduction of ROS by upregulating anti-oxidant defense genes through Nrf2 activation and SIRT-1 expression. Concerning inflammatory responses, resveratrol suppressed the 5-FU-induced expression of pro-inflammatory cytokines via NF-κB nuclear translocation. Conversely, N-acetylcysteine reduced ROS levels without affecting the expression of pro-inflammatory cytokines. Resveratrol might be useful for preventing 5-FU-induced adverse effects by activating anti-oxidant and anti-inflammatory responses.
• DOI: info:doi/10.3164/jcbn.21-23; https://doi.org/info:doi/10.3164/jcbn.21-23
• Persistent ID (NDL): info:ndljp/pid/11864783; https://dl.ndl.go.jp/pid/11864783
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2021-11-08T14:10:24+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/116380
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp