Alternative TitleHuman Cathelicidin Antimicrobial Peptide LL-37 Promotes Lymphangiogenesis in Lymphatic Endothelial Cells through the ERK and Akt Signaling Pathways
Degree Type博士(歯学)
Doctor of Philosophy in Dental Science
Note (General)博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨
LL-37, the only member of the cathelicidin family of cationic antimicrobial peptides in humans has been shown to exhibit a wide variety of biological actions in addition to its antimicrobial activity. However, the lymphangiogenic effect of LL-37 has not been elucidated yet. In this study, we examined the effects of LL-37 on lymphangiogenesis and evaluated the underlying molecular mechanisms. LL-37 treatment significantly increased the migration and tube-like formation of human dermal lymphatic microvascular endothelial cells (HDLECs) and promoted the expression of lymphangiogenic factor in HDLECs. Treatment with LL-37 increased phosphorylation of ERK and Akt proteins in HDLECs, and pretreatment with ERK and Akt inhibitors significantly blocked the LL-37-induced HDLEC migration and tube-like formation. Furthermore, to investigate the involvement of formyl peptide receptor-like 1 (FPRL1) signaling in LL-37-induced lymphangiogenesis, HDLECs were treated with an FPRL1 antagonist. Pretreatment with the FPRL1 antagonist inhibited LL-37-induced phosphorylation of ERK and Akt proteins and attenuated LL-37-induced HDLEC migration and tube-like formation. These data indicated that LL-37 induces lymphangiogenesis in lymphatic endothelial cells via FPRL1, and the activation of the ERK and Akt-dependent signaling pathways.Takahiro Yanagisawa, Masakazu Ishii, Manami Takahashi, Kei Fujishima & Masahiro NishimuraHuman cathelicidin antimicrobial peptide LL-37 promotes lymphangiogenesis in lymphatic endothelial cells through the ERK and Akt signaling pathwaysMolecular Biology Reports volume 47, pages6841–6854 (2020)This is a post-peer-review, pre-copyedit version of an article published in Molecular Biology Reports .The final authenticated version is available online at:https://doi.org/10.1007/s11033-020-05741-8
Collection (particular)国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
Date Accepted (W3CDTF)2021-11-08T14:10:24+09:00
Data Provider (Database)国立国会図書館 : 国立国会図書館デジタルコレクション