Combination of RERG and ZNF671 methylation rates in circulating cell-free DNA: A novel biomarker for screening of nasopharyngeal carcinoma

• Material Type: 博士論文
• Title: Combination of RERG and ZNF671 methylation rates in circulating cell-free DNA: A novel biomarker for screening of nasopharyngeal carcinoma
• Author/Editor: Xu, Yifei
• Author Heading: Xu, Yifei
• Publication, Distribution, etc.: 三重大学
• Publication Date: 2021-09-15
• Publication Date (W3CDTF): 2021-09-15
• Degree grantor/type: 三重大学
• Date Granted: 2021-09-15
• Date Granted (W3CDTF): 2021-09-15
• Dissertation Number: 甲医学第2088号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲医学第2088号
• Text Language Code: eng
• Subject Heading: circulating cell-free DNA; DNA methylation; nasopharyngeal carcinoma; qAMP; screening biomarker
• Target Audience: 一般
• Note (General): application/pdf; Nasopharyngeal carcinoma (NPC) is a prevalent malignancy in Southeast Asia, hence, identifying easily detectable biomarkers for NPC screening is essential for better diagnosis and prognosis. Using genome-wide and targeted analyses based on next-generation sequencing approaches, we previously showed that gene promoters are hypermethylated in NPC tissues. To confirm whether DNA methylation rates of genes could be used as biomarkers for NPC screening, 79 histologically diagnosed NPC patients and 29 noncancer patients were recruited. A convenient quantitative analysis of DNA methylation using real-time PCR (qAMP) was carried out, involving pretreatment of tissue DNA, and circulating cell-free DNA (ccfDNA) from nonhemolytic plasma, with methylation-sensitive and/or methylation-dependent restriction enzymes. The qAMP analyses revealed that methylation rates of RERG, ZNF671, ITGA4, and SHISA3 were significantly higher in NPC primary tumor tissues compared to noncancerous tissues, with sufficient diagnostic accuracy of the area under receiver operating characteristic curves (AUC). Interestingly, higher methylation rates of RERG in ccfDNA were statistically significant and yielded a very good AUC; however, those of ZNF671, ITGA4, and SHISA3 were not significant. Furthermore, the combination of methylation rates of RERG and ZNF671 in ccfDNA showed higher diagnostic accuracy than either of them individually. In conclusion, the methylation rates of specific genes in ccfDNA can serve as novel biomarkers for early detection and screening of NPC.; 本文/Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine, Tsu, Japan; 10p
• DOI: info:doi/10.1111/cas.14431; https://doi.org/info:doi/10.1111/cas.14431
• Persistent ID (NDL): info:ndljp/pid/11976675; https://dl.ndl.go.jp/pid/11976675
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2022-01-10T16:22:37+09:00
• Date Created (W3CDTF): 2022-01-04
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
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• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10076/00020072; https://mie-u.repo.nii.ac.jp/records/14454
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp