Intestinal Epithelium-Derived Luminally Released Extracellular Vesicles in Sepsis Exhibit the Ability to Suppress TNF-α and IL-17A Expression in Mucosal Inflammation

• Material Type: 博士論文
• Title: Intestinal Epithelium-Derived Luminally Released Extracellular Vesicles in Sepsis Exhibit the Ability to Suppress TNF-α and IL-17A Expression in Mucosal Inflammation
• Author/Editor: Appiah, Michael Gyasi
• Author Heading: Appiah, Michael Gyasi
• Publication, Distribution, etc.: 三重大学
• Publication Date: 2021-09-15
• Publication Date (W3CDTF): 2021-09-15
• Degree grantor/type: 三重大学
• Date Granted: 2021-09-15
• Date Granted (W3CDTF): 2021-09-15
• Dissertation Number: 甲医学第2083号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲医学第2083号
• Text Language Code: eng
• Subject Heading: sepsis; intestinal epithelial cells; inflammation; extracellular vesicles; TNF-α; IL-17A; miRNAs
• Target Audience: 一般
• Note (General): application/pdf; Sepsis is a systemic inflammatory disorder induced by a dysregulated immune response to infection resulting in dysfunction of multiple critical organs, including the intestines. Previous studies have reported contrasting results regarding the abilities of exosomes circulating in the blood of sepsis mice and patients to either promote or suppress inflammation. Little is known about how the gut epithelial cell-derived exosomes released in the intestinal luminal space during sepsis affect mucosal inflammation. To study this question, we isolated extracellular vesicles (EVs) from intestinal lavage of septic mice. The EVs expressed typical exosomal (CD63 and CD9) and epithelial (EpCAM) markers, which were further increased by sepsis. Moreover, septic-EV injection into inflamed gut induced a significant reduction in the messaging of pro-inflammatory cytokines TNF-a and IL-17A. MicroRNA (miRNA) profiling and reverse transcription and quantitative polymerase chain reaction (RT-qPCR) revealed a sepsis-induced exosomal increase in multiple miRNAs, which putatively target TNF-a and IL-17A. These results imply that intestinal epithelial cell (IEC)-derived luminal EVs carry miRNAs that mitigate pro-inflammatory responses. Taken together, our study proposes a novel mechanism by which IEC EVs released during sepsis transfer regulatory miRNAs to cells, possibly contributing to the amelioration of gut inflammation.; 本文/Department of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, Japan; 44p
• DOI: info:doi/10.3390/ijms21228445; https://doi.org/info:doi/10.3390/ijms21228445
• Persistent ID (NDL): info:ndljp/pid/11976679; https://dl.ndl.go.jp/pid/11976679
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2022-01-10T16:22:37+09:00
• Date Created (W3CDTF): 2022-01-04
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10076/00020068; https://mie-u.repo.nii.ac.jp/records/14450
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp