Inhibitory effect of HTLV-1 infection on the production of B-cell activating factors in established follicular dendritic cell-like cells

• Material Type: 博士論文
• ISSN: 2050-4527
• Title: Inhibitory effect of HTLV-1 infection on the production of B-cell activating factors in established follicular dendritic cell-like cells
• Volume: 9 3
• Author/Editor: 髙谷, 亜由子
• Author Heading: 髙谷, 亜由子
• Publication, Distribution, etc.: Wiley & Sons Ltd.
• Publication Date: 2021-06-02
• Publication Date (W3CDTF): 2021-06-02
• Alternative Title: 濾胞性樹状細胞様細胞のB細胞活性化因子産生に対するHTLV-1感染の抑制効果
• Periodical title: Immunity, Inflammation and Disease
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2021-06-02
• Date Granted (W3CDTF): 2021-06-02
• Dissertation Number: 甲共医歯薬第12号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲共医歯薬第12号
• Text Language Code: eng
• Subject Heading: B‐cell activating factor; FDC; follicular dendritic cell; HTLV-1; Sjögren's syndrome
• Target Audience: 一般
• Note (General): Introduction: The low frequency of ectopic germinal center in labial salivary glands of patients with human T-cell leukemia virus type 1 (HTLV-1) antibody-positive Sjögren's syndrome (SS) suggests that HTLV-1 has some effects on follicular dendritic cells (FDCs).Methods: We used flow cytometry, immunofluorescence, and enzyme-linked immunosorbent assays (ELISAs) to investigate the direct effect of HTLV-1 on B-cell activating factors produced by established FDC like cells obtained from excised human tonsils. We then measured the serum B-cell activating factor (BAFF) and C-X-C motif ligand (CXCL) 13 concentrations of the HTLV-1-seropositive SS patients and the HTLV-1-seronegative SS patients by ELISA.Results: Among the 31 isolated specimens, 22 showed morphological characteristics of FDCs. Day 2-cultured specimens showed expressions of CD14, CD23, CD40, intracellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1. After 2 weeks, 12 of these specimens expressed ICAM-1, FDC, and fibroblast cell marker. Intracellular BAFF and CXCL13 were constitutively expressed regardless of stimulation. After direct coculture with the HTLV-1-infected T-cell line HCT-5 or MT-2, the BAFF and CXCL13 expressions on the FDC-like cells were decreased in accord with the increased number of HCT-5 and MT-2 cells with styliform change and without HTLV-1 Gag protein expression. Interferons upregulated the concentration of BAFF (but not CXCL13) in the culture supernatant, which showed a declining trend under the presence of HCT-5 or MT-2. The serum concentrations of BAFF and CXCL13 in the HTLV-1-seropositive SS patients were lower than those of the HTLV-1 seronegative SS patients.Conclusions: HTLV-1 partially inhibited the BAFF and CXCL13 expressions of established FDC-like cells; 長崎大学学位論文 学位記番号:共博（医歯薬）甲第12号 学位授与年月日:令和3年6月2日; Author: Ayuko Takatani, Hideki Nakamura, Kaori Furukawa, Yushiro Endo, Masataka Umeda, Toshimasa Shimizu, Shin‐ya Nishihata, Kyoko Kitaoka, Tatsufumi Nakamura, Atsushi Kawakami; Citation: Immunity, Inflammation and Disease, 9(3), pp. 777-791; 2021; identifier:Nagasaki University (長崎大学), 博士(医学) (2021-06-02); http://hdl.handle.net/10069/00040842
• DOI: info:doi/10.1002/iid3.432; https://doi.org/info:doi/10.1002/iid3.432
• Persistent ID (NDL): info:ndljp/pid/11976687; https://dl.ndl.go.jp/pid/11976687
• Collection: 障害者向け資料
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• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2022-01-10T16:22:37+09:00
• Date Created (W3CDTF): 2022-01-04
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
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• Related Material (URI): http://hdl.handle.net/10069/00040842
• Periodical Title (URI): http://hdl.handle.net/10069/00041134
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp