Human Parainfluenza Virus Type 2 Vector Induces Dendritic Cell Maturation Without Viral RNA Replication/Transcription

Persistent ID (NDL): info:ndljp/pid/11977086

Material type: 博士論文

Author: Hara, Kenichiro

Publisher: 三重大学

Publication date: 2014-03-25

Material Format: Digital

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Name of awarding university/degree: 三重大学,博士(医学)

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Note (General)：: application/pdfThe dendritic cell (DC), a most potent antigen-presenting cell, plays a key role in vaccine therapy against infectious diseases and mal...

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Material Type: 博士論文
Title: Human Parainfluenza Virus Type 2 Vector Induces Dendritic Cell Maturation Without Viral RNA Replication/Transcription
Author/Editor: Hara, Kenichiro
Author Heading: Hara, Kenichiro
Publication, Distribution, etc.: 三重大学
Publication Date: 2014-03-25
Publication Date (W3CDTF): 2014-03-25
Degree grantor/type: 三重大学
Date Granted: 2014-03-25
Date Granted (W3CDTF): 2014-03-25
Dissertation Number: 甲医学第1656号
Degree Type: 博士(医学)
Conferring No. (Dissertation): 甲医学第1656号
Text Language Code: eng
Alias of Author: ハラ, ケンイチロウ
原, 健一郎
Target Audience: 一般
Note (General): application/pdf
The dendritic cell (DC), a most potent antigen-presenting cell, plays a key role in vaccine therapy against infectious diseases and malignant tumors. Although advantages of viral vectors for vaccine therapy have been reported, potential risks for adverse effects prevent them from being licensed for clinical use. Human parainfluenza virus type 2 (hPIV2), one of the members of the Paramyxoviridae family, is a nonsegmented and negative-stranded RNA virus. We have developed a reverse genetics system for the production of infectious hPIV2 lacking the F gene (hPIV2ΔF), wherein various advantages for vaccine therapy exist, such as cytoplasmic replication/transcription, nontransmissible infectivity, and extremely high transduction efficacy in various types of target cells. Here we demonstrate that hPIV2ΔF shows high transduction efficiency in human DCs, while not so high in mouse DCs. In addition, hPIV2ΔF sufficiently induces maturation of both human and murine DCs, and the maturation state of both human and murine DCs is almost equivalent to that induced by lipopolysaccharide. Moreover, alkylating agent β-propiolactone-inactivated hPIV2ΔF (BPL-hPIV2ΔF) elicits DC maturation without viral replication/transcription. These results suggest that hPIV2ΔF may be a useful tool for vaccine therapy as a novel type of paramyxoviral vector, which is single-round infectious vector and has potential adjuvant activity.
本文/Department of Microbiology and Molecular Genetics, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, Japan.
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DOI: info:doi/10.1089/hum.2013.024
https://doi.org/info:doi/10.1089/hum.2013.024
Persistent ID (NDL): info:ndljp/pid/11977086
https://dl.ndl.go.jp/pid/11977086
Collection: 障害者向け資料
Collection (Materials For Handicapped People:1): テキストデータ
Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
https://dl.ndl.go.jp/collections/A00014
Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2022-01-10T16:22:37+09:00
Date Created (W3CDTF): 2021-12-09
Format (IMT): application/pdf
Access Restrictions: 国立国会図書館内限定公開
Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
Availability of remote photoduplication service: 可
Periodical Title (URI): http://hdl.handle.net/10076/00020031
https://mie-u.repo.nii.ac.jp/records/14413
Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション
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