In vivo optical pathology of paclitaxel efficacy on the peritoneal metastatic xenograft model of gastric cancer using two-photon laser scanning microscopy

• Material Type: 博士論文
• Title: In vivo optical pathology of paclitaxel efficacy on the peritoneal metastatic xenograft model of gastric cancer using two-photon laser scanning microscopy
• Author/Editor: Shimura, Tadanobu
• Author Heading: Shimura, Tadanobu
• Publication, Distribution, etc.: 三重大学
• Publication Date: 2014-03-25
• Publication Date (W3CDTF): 2014-03-25
• Degree grantor/type: 三重大学
• Date Granted: 2014-03-25
• Date Granted (W3CDTF): 2014-03-25
• Dissertation Number: 甲医学第1654号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲医学第1654号
• Text Language Code: eng
• Alias of Author: シムラ, タダノブ; 志村, 匡信
• Subject Heading: Paclitaxel; Peritoneal metastatic xenograft; Gastric cancer; Two-photon laser scanning microscopy
• Target Audience: 一般
• Note (General): application/pdf; Background We previously visualized in vivo responses to chemotherapy in a colorectal liver metastatic xenograft model using in vivo real-time and time-series intravital two-photon laser scanning microscopy (TPLSM). In this study, we established the method for evaluating the response of peritoneal xenografts to chemotherapy of metastatic gastric cancer using intravital TPLSM.Methods Red fluorescent protein-expressing gastric cancer cells (NUGC4) were inoculated into the peritoneal cavity of green fluorescent protein nude mice.Results Laparotomy revealed that 2 weeks after inoculation, macroscopic peritoneal metastatic nodules were formed. The first intravital TPLSM session revealed that they were composed of red tumor cell clusters and green surrounding stroma. Paclitaxel was administered intraperitoneally after the first TPLSM three times a week for 7 days in the treatment group. At the second laparotomy, there were significantly fewer and smaller nodules in the treated mice than in the controls. The second intravital TPLSM session showed tumor cell fragmentation, swelling, and nuclear condensation in the metastatic nodules—a response to chemotherapy. There were multinuclear tumor cells in the paclitaxel-treated living mice.Conclusions Our method may become a powerful tool for evaluating the efficacy of novel anti-gastric cancer drugs in a preclinical murine model with minimum interindividual variation.; 本文/Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan; 10p
• DOI: info:doi/10.1007/s10120-013-0334-y; https://doi.org/info:doi/10.1007/s10120-013-0334-y
• Persistent ID (NDL): info:ndljp/pid/11977088; https://dl.ndl.go.jp/pid/11977088
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2022-01-10T16:22:37+09:00
• Date Created (W3CDTF): 2021-12-09
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10076/00020029; https://mie-u.repo.nii.ac.jp/records/14411
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp