Cyclic compressive mechanical loading on three-dimensional cultured tissue of human chondrocytes synergistically upregulates MMP-3 gene expression with IL-1β

Persistent ID (NDL): info:ndljp/pid/12071390

Material type: 博士論文

Author: 疋田, 光波

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Publication date: 2021-03-05

Material Format: Digital

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Name of awarding university/degree: 大阪歯科大学,博士（歯学）

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Note (General)：: Matrix proteases are involved in the destruction and degeneration of articular cartilage, which is one of the pathological conditions of osteoarthriti...

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Material Type: 博士論文
Title: Cyclic compressive mechanical loading on three-dimensional cultured tissue of human chondrocytes synergistically upregulates MMP-3 gene expression with IL-1β
Author/Editor: 疋田, 光波
Author Heading: 疋田, 光波
Publication Date: 2021-03-05
Publication Date (W3CDTF): 2021-03-05
Alternative Title: IL-1β刺激下のヒト軟骨細胞三次元組織への繰り返し圧縮負荷はMMP-3の遺伝子発現を相乗的に増加させる
Degree grantor/type: 大阪歯科大学
Date Granted: 2021-03-05
Date Granted (W3CDTF): 2021-03-05
Dissertation Number: 甲第907号
Degree Type: 博士（歯学）
Conferring No. (Dissertation): 甲第907号
Text Language Code: eng
Subject Heading: Three-dimensional tissue
Human chondrocytes
Matrix proteinase
Cyclic compression loading
Target Audience: 一般
Note (General): Matrix proteases are involved in the destruction and degeneration of articular cartilage, which is one of the pathological conditions of osteoarthritis of the temporomandibular joint (TMJOA). We investigated the expression of matrix proteases in combination with IL-1β and cyclic compression loading on three-dimensional (3D) cultured tissues of human articular chondrocytes. Human articular chondrocytes were seeded in atelocollagen sponge and cultured in 3D. Cyclic compressive loading of 40 kPa at 0.5 Hz was applied to the constructs for 1 h, with or without the administration of IL-1β, which was added at concentrations of 0.1, 1 and 10 ng/mL. After 6 or 12 h of cyclic compression loading, the gene expression of the matrix proteases were evaluated by qRT-PCR. We found that cyclic compression loading significantly increased the expression of MMP-3, ADAMTS-4 and ADAM-17, while IL-1β stimulation significantly increased the expression of MMP-3, MMP-13, ADAMTS-4, ADAMTS-5 and ADAM-17. The expression of MMP-3 was synergistically upregulated by combining cyclic compressive loading with IL-1β when compared with a single stimulation. Therefore we believe that the application of our present biomechanical and biochemical model, which mimics the human intra-articular environment of OA, can facilitate elucidation of the mechanisms of articular cartilage degeneration and destruction in TMJOA.
2020年度
Persistent ID (NDL): info:ndljp/pid/12071390
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Collection: 障害者向け資料
Collection (Materials For Handicapped People:1): テキストデータ
Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
https://dl.ndl.go.jp/collections/A00014
Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2022-02-06T04:33:19+09:00
Date Created (W3CDTF): 2021-04-23
Format (IMT): application/pdf
Access Restrictions: 国立国会図書館内限定公開
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Availability of remote photoduplication service: 可
Periodical Title (URI): http://id.nii.ac.jp/1392/00000274/
Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション
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