Detection of exosomal miRNA in GCF with chronic periodontitis

Persistent ID (NDL): info:ndljp/pid/12071400

Material type: 博士論文

Author: 河東, 里奈

Publisher: -

Publication date: 2021-03-05

Material Format: Digital

Capacity, size, etc.: -

Name of awarding university/degree: 大阪歯科大学,博士（歯学）

View All

Notes on use at the National Diet Library

本資料は、掲載誌(URI)等のリンク先にある学位授与機関のWebサイトやCiNii Dissertationsから、本文を自由に閲覧できる場合があります。

Notes on use

Note (General)：: MicroRNAs suppress the expression of target genes and extensively regulate the expression of genes involved in growth and disease. The purpose of this...

Search by Bookstore

Read this material in an accessible format.

View materials in accessible formats for people with print disabilities (1Type)

Search by Bookstore

Read in Disability Resources

Other Services
- テキストデータ check it on 国立国会図書館デジタルコレクション

Bibliographic Record

You can check the details of this material, its authority (keywords that refer to materials on the same subject, author's name, etc.), etc.

Digital

Material Type: 博士論文
Title: Detection of exosomal miRNA in GCF with chronic periodontitis
Author/Editor: 河東, 里奈
Author Heading: 河東, 里奈
Publication Date: 2021-03-05
Publication Date (W3CDTF): 2021-03-05
Alternative Title: 慢性歯周炎特有の歯肉溝滲出液中エクソソームにおけるmiRNAの検出
Degree grantor/type: 大阪歯科大学
Date Granted: 2021-03-05
Date Granted (W3CDTF): 2021-03-05
Dissertation Number: 甲第897号
Degree Type: 博士（歯学）
Conferring No. (Dissertation): 甲第897号
Text Language Code: eng
Subject Heading: MicroRNA
Exosome
Periodontal disease
Gingival crevicular fluid
Target Audience: 一般
Note (General): MicroRNAs suppress the expression of target genes and extensively regulate the expression of genes involved in growth and disease. The purpose of this study was to detect exosomes from gingival crevicular fluid (GCF), and to elucidate disease-specific exosomal miRNA expression in GCF from patients with chronic periodontal disease. Comprehensive microRNA expression analysis was performed using exosomal miRNA in GCF to compare the GCF of patients with chronic periodontitis disease with that of healthy participants. The candidate exosomal miRNAs were analyzed using real-time quantitative polymerase chain reaction (qPCR). The presence of exosomes was observed by measuring the particle size, as well as by detecting CD9/CD63 and observing transmission electron microscope images (TEM). Eleven exosomal miRNAs were identified as differentially expressed more than 8 times lower than in healthy participants by global analysis. Of the miRNA, qPCR analysis revealed that the expression of hsa-mir-204-3p was lower in GCF collected from affected and unaffected sites of patients with chronic periodontal disease, compared with those collected from healthy individuals. This suggests that hsa-mir-204-3p might be associated with periodontal pathology.
2020年度
Persistent ID (NDL): info:ndljp/pid/12071400
https://dl.ndl.go.jp/pid/12071400
Collection: 障害者向け資料
Collection (Materials For Handicapped People:1): テキストデータ
Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
https://dl.ndl.go.jp/collections/A00014
Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2022-02-06T04:33:19+09:00
Date Created (W3CDTF): 2021-04-23
Format (IMT): application/pdf
Access Restrictions: 国立国会図書館内限定公開
Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
Availability of remote photoduplication service: 可
Periodical Title (URI): http://id.nii.ac.jp/1392/00000264/
Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション
https://dl.ndl.go.jp