Expression of Heat Shock Proteins in Response to Mild Short-term Heat Shock in Human Deciduous Dental Pulp Fibroblast-like Cells

Persistent ID (NDL): info:ndljp/pid/12071402

Material type: 博士論文

Author: 青木, 翔

Publisher: -

Publication date: 2021-03-05

Material Format: Digital

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Name of awarding university/degree: 大阪歯科大学,博士（歯学）

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Note (General)：: Appropriate heat shock results in the production of heat shock proteins (HSPs) whose expression and phosphoryl- ation contribute to repair of damaged ...

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Material Type: 博士論文
Title: Expression of Heat Shock Proteins in Response to Mild Short-term Heat Shock in Human Deciduous Dental Pulp Fibroblast-like Cells
Author/Editor: 青木, 翔
Author Heading: 青木, 翔
Publication Date: 2021-03-05
Publication Date (W3CDTF): 2021-03-05
Alternative Title: ヒト乳歯歯髄由来線維芽細胞における軽度・短時間の熱刺激によるHeat shock proteins発現
Degree grantor/type: 大阪歯科大学
Date Granted: 2021-03-05
Date Granted (W3CDTF): 2021-03-05
Dissertation Number: 甲第895号
Degree Type: 博士（歯学）
Conferring No. (Dissertation): 甲第895号
Text Language Code: eng
Subject Heading: Human deciduous dental pulp fibroblast-like cells｜Heat shock proteins
Target Audience: 一般
Note (General): Appropriate heat shock results in the production of heat shock proteins (HSPs) whose expression and phosphoryl- ation contribute to repair of damaged proteins, cell proliferation, and cell recovery from shock stimuli. However, there is no information regarding the expression of HSPs in human deciduous dental pulp fibroblast-like cells (hDDPF) in response to mild short-term heat shock. The aim of this study was to investigate the cellular effects of mild short-term heat shock on hDDPF. Cells were subjected to heat shock at 43°C–49°C for 15 min, and cell proliferation was assessed by 3-(4,5-dimeth- ylthiazol-2yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay. mRNA and protein expressions of HSP27, 70, and 90 were detected by reverse transcription PCR and western blot analysis, respectively. Phosphorylation of the AKT and ERK signaling pathways of HSP production was evaluated by western blotting. Heat shock at 43°C for 15 min increased the cell proliferation rate and the mRNA expressions of HSP27, 70, and 90 in hDDPF. Moreover, protein expres- sion of HSP70 was significantly enhanced 24 h after heat shock, and the phosphorylation of AKT was also confirmed. Be- cause HSP70 is critical in tissue repair and regeneration, mild short-term heat shock may enhance tissue repair in hDDPF.
2020年度
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Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
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Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2022-02-06T04:33:19+09:00
Date Created (W3CDTF): 2021-04-23
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Periodical Title (URI): http://id.nii.ac.jp/1392/00000262/
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