Effect of mussel adhesive protein coating on osteogenesis in vitro and osteointegration in vivo to alkali-treated titanium with nanonetwork structures

• Material Type: 博士論文
• Title: Effect of mussel adhesive protein coating on osteogenesis in vitro and osteointegration in vivo to alkali-treated titanium with nanonetwork structures
• Author/Editor: 尹, 德荣
• Author Heading: 尹, 德荣
• Publication Date: 2020-03-06
• Publication Date (W3CDTF): 2020-03-06
• Alternative Title: イガイ接着タンパク質のコーティングがナノ構造析出純チタンへの生体外骨形成と生体内オッセオインテグレーションに与える影響
• Degree grantor/type: 大阪歯科大学
• Date Granted: 2020-03-06
• Date Granted (W3CDTF): 2020-03-06
• Dissertation Number: 甲第883号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第883号
• Text Language Code: eng
• Subject Heading: biocomposite｜bone marrow mesenchymal stem cell｜extracellular matrix｜nanopore｜polydopamine
• Target Audience: 一般
• Note (General): Purpose: On the basis of reasonable superposition of various surface treatment methods, alkali-treated titanium with nanonetwork structures (TNS) was coated with mussel adhesive protein (MAP) and named TNS-MAP. The aims were to optimize the biological properties of TNS, endue it with new properties, and enhance its utility in clinical dental applications. Methods: TNS disks were coated with MAP and the product surface was characterized. Its osteogenic properties were determined by evaluating its effects on cell adhesion, cell proliferation, the expression of osteogenesis-related genes, and in vivo experiments. Results: The treated materials showed excellent hydrophilicity, good surface roughness, and advantages of both TNS and MAP. TNS-MAP significantly promoted initial cell attachment especially after 15 mins and 30 mins. At every time point, cell adhesion and proliferation, the detection rate of osteogenesis-related markers in the extracellular matrix, and the expression of osteogenesis-related genes were markedly superior on TNS-MAP than the control. The in vivo experiments revealed that TNS-MAP promoted new bone growth around the implants and the bone–implant interface. Conclusion: We verified through in vitro and in vivo experiments that we successfully created an effective TNS-MAP composite implant with excellent biocompatibility and advantages of both its TNS and MAP parent materials. Therefore, the new biocomposite implant material TNS-MAP may potentially serve in practical dentistry and orthopedics.; 2019年度
• Persistent ID (NDL): info:ndljp/pid/12071494; https://dl.ndl.go.jp/pid/12071494
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2022-02-06T04:33:19+09:00
• Date Created (W3CDTF): 2020-04-28
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://id.nii.ac.jp/1392/00000246/
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp