Palmitate induces apoptosis and inhibits osteogenic differentiation of human periodontal ligament stem cells

Persistent ID (NDL): info:ndljp/pid/12071499

Material type: 博士論文

Author: 竹内, 友規

Publisher: -

Publication date: 2020-03-06

Material Format: Digital

Capacity, size, etc.: -

Name of awarding university/degree: 大阪歯科大学,博士（歯学）

View All

Notes on use at the National Diet Library

本資料は、掲載誌(URI)等のリンク先にある学位授与機関のWebサイトやCiNii Dissertationsから、本文を自由に閲覧できる場合があります。

Notes on use

Note (General)：: Objective: The aim of the present study was to investigate the effect of palmitate on human periodontal ligament stem cells (PDLSCs).Design: PDLSCs we...

Search by Bookstore

Read this material in an accessible format.

View materials in accessible formats for people with print disabilities (1Type)

Search by Bookstore

Read in Disability Resources

Other Services
- テキストデータ check it on 国立国会図書館デジタルコレクション

Bibliographic Record

You can check the details of this material, its authority (keywords that refer to materials on the same subject, author's name, etc.), etc.

Digital

Material Type: 博士論文
Title: Palmitate induces apoptosis and inhibits osteogenic differentiation of human periodontal ligament stem cells
Author/Editor: 竹内, 友規
Author Heading: 竹内, 友規
Publication Date: 2020-03-06
Publication Date (W3CDTF): 2020-03-06
Alternative Title: パルミチン酸はヒト歯根膜幹細胞のアポトーシスを誘導し、骨芽細胞分化を阻害する
Degree grantor/type: 大阪歯科大学
Date Granted: 2020-03-06
Date Granted (W3CDTF): 2020-03-06
Dissertation Number: 甲第878号
Degree Type: 博士（歯学）
Conferring No. (Dissertation): 甲第878号
Text Language Code: eng
Subject Heading: Periodontal ligament
Stem cells
Palmitate
Apoptosis
Osteogenesis
Target Audience: 一般
Note (General): Objective: The aim of the present study was to investigate the effect of palmitate on human periodontal ligament stem cells (PDLSCs).Design: PDLSCs were isolated from the third molars of healthy adult donors, and cultured in normal or osteogenic medium supplemented with palmitate (0, 100, or 250 µM) for 21 days. Cell proliferation was evaluated by measuring the amount of formazan at 6, 24, 48, and 72 h. Apoptosis was detected by ELISA and terminal deoxynucleotidyl transferase dUTP nick end labeling assay at days 3 and 7. Osteogenic differentiation was evaluated by measuring the alkaline phosphatase (ALP) activity, production of procollagen type I C-peptide and osteocalcin, mineralization, and mRNA expression of Runx2 at days 3, 7, 14, and 21. In addition, mRNA expression of IL-6 and IL-8 was measured at day 3.Results: Palmitate inhibited the proliferation, ALP activity, production of procollagen type I C-peptide and osteocalcin, mineralization, and mRNA expression of Runx2 in the cultured PDLSCs. Palmitate also induced apoptosis and mRNA expression of IL-6 and IL-8 in the PDLSCs.Conclusions: The results of the present study demonstrate that palmitate induces apoptosis and inhibits osteogenic differentiation of PDLSCs. These findings may help clarify the relationship between palmitate and periodontal tissue regeneration.
2019年度
Persistent ID (NDL): info:ndljp/pid/12071499
https://dl.ndl.go.jp/pid/12071499
Collection: 障害者向け資料
Collection (Materials For Handicapped People:1): テキストデータ
Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
https://dl.ndl.go.jp/collections/A00014
Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2022-02-06T04:33:19+09:00
Date Created (W3CDTF): 2020-04-28
Format (IMT): application/pdf
Access Restrictions: 国立国会図書館内限定公開
Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
Availability of remote photoduplication service: 可
Periodical Title (URI): http://id.nii.ac.jp/1392/00000241/
Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション
https://dl.ndl.go.jp