Impact of short-term saliva storage at room temperature on the microbial composition

Persistent ID (NDL): info:ndljp/pid/12071514

Material type: 博士論文

Author: 榎本, 温子

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Publication date: 2020-03-06

Material Format: Digital

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Name of awarding university/degree: 大阪歯科大学,博士（歯学）

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Note (General)：: Large-scale cohort studies using saliva samples have been performed to elucidate the relationship between oral and systemic health and oral resident m...

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Material Type: 博士論文
Title: Impact of short-term saliva storage at room temperature on the microbial composition
Author/Editor: 榎本, 温子
Author Heading: 榎本, 温子
Publication Date: 2020-03-06
Publication Date (W3CDTF): 2020-03-06
Alternative Title: 唾液の室温での短期保存による微生物組成への影響
Degree grantor/type: 大阪歯科大学
Date Granted: 2020-03-06
Date Granted (W3CDTF): 2020-03-06
Dissertation Number: 甲第863号
Degree Type: 博士（歯学）
Conferring No. (Dissertation): 甲第863号
Text Language Code: eng
Subject Heading: saliva｜ microbiota｜ 16S rRNA gene｜storage
Target Audience: 一般
Note (General): Large-scale cohort studies using saliva samples have been performed to elucidate the relationship between oral and systemic health and oral resident microbiota. Although standardization of the sampling and processing procedures for saliva that is used in next-generation sequencing (NGS) analysis is underway, the storage conditions after collecting saliva have not been studied in detail. In this study, we monitored changes in the salivary microbiota during short-term storage at room temperature. Saliva samples were collected from 11 subjects and stored at room temperature (23°C) for 3 hours. When the V3–V4 region of the 16S rRNA gene was analyzed using NGS, there was no change in the β-diversity of salivary bacterial microbiota, but a significant decrease in α-diversity was observed. Additionally, a significant decrease was observed in four operational taxonomic units (OTUs). These results suggest that even short-term storage of saliva samples at room temperature may affect the diversity of salivary microbiota and the relative abundance of specific OTUs.
2019年度
Persistent ID (NDL): info:ndljp/pid/12071514
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Collection: 障害者向け資料
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Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2022-02-06T04:33:19+09:00
Date Created (W3CDTF): 2020-04-28
Format (IMT): application/pdf
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Periodical Title (URI): http://id.nii.ac.jp/1392/00000226/
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