Osteogenesis of Multipotent Progenitor Cells using the Epigallocatechin Gallate-Modified Gelatin Sponge Scaffold in the Rat Congenital Cleft-Jaw Model

Persistent ID (NDL): info:ndljp/pid/12071549

Material type: 博士論文

Author: 笹山, 智史

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Publication date: 2019-03-08

Material Format: Digital

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Name of awarding university/degree: 大阪歯科大学,博士（歯学）

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Note (General)：: Cost-effective and functionalized scaffolds are in high demand for stem-cell-based regenerative medicine to treat refractory bone defects in craniofac...

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Material Type: 博士論文
Title: Osteogenesis of Multipotent Progenitor Cells using the Epigallocatechin Gallate-Modified Gelatin Sponge Scaffold in the Rat Congenital Cleft-Jaw Model
Author/Editor: 笹山, 智史
Author Heading: 笹山, 智史
Publication Date: 2019-03-08
Publication Date (W3CDTF): 2019-03-08
Alternative Title: ラット先天性顎裂モデルにおける多能性前駆細胞を播種したEGCG結合ゼラチンの骨形成
Degree grantor/type: 大阪歯科大学
Date Granted: 2019-03-08
Date Granted (W3CDTF): 2019-03-08
Dissertation Number: 甲第838号
Degree Type: 博士（歯学）
Conferring No. (Dissertation): 甲第838号
Text Language Code: eng
Subject Heading: plant polyphenol｜ EGCG｜ gelatin｜bone formation｜congenital bone defect｜dedifferentiated fat cell｜adipose-derived stem cell｜scaffold
Target Audience: 一般
Note (General): Cost-effective and functionalized scaffolds are in high demand for stem-cell-based regenerative medicine to treat refractory bone defects in craniofacial abnormalities and injuries. One potential strategy is to utilize pharmacological and cost-effective plant polyphenols and biocompatible proteins, such as gelatin. Nevertheless, the use of chemically modified proteins with plant polyphenols in this strategy has not been standardized. Here, we demonstrated that gelatin chemically modified with epigallocatechin gallate (EGCG), the major catechin isolated from green tea, can be a useful material to induce bone regeneration in a rat congenial cleft-jaw model in vivo when used with/without adipose-derived stem cells or dedifferentiated fat cells. Vacuum-heated gelatin sponges modified with EGCG (vhEGCG-GS) induced superior osteogenesis from these two cell types compared with vacuum-heated gelatin sponges (vhGS). The EGCG-modification converted the water wettability of vhGS to a hydrophilic property (contact angle: 110° to 3.8°) and the zeta potential to a negative surface charge; the modification enhanced the cell adhesion property and promoted calcium phosphate precipitation. These results suggest that the EGCG-modification with chemical synthesis can be a useful platform to modify the physicochemical property of gelatin. This alteration is likely to provide a preferable microenvironment for multipotent progenitor cells, inducing superior bone formation in vivo.
2018年度
Persistent ID (NDL): info:ndljp/pid/12071549
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Collection: 障害者向け資料
Collection (Materials For Handicapped People:1): テキストデータ
Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
https://dl.ndl.go.jp/collections/A00014
Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2022-02-06T04:33:19+09:00
Date Created (W3CDTF): 2019-05-17
Format (IMT): application/pdf
Access Restrictions: 国立国会図書館内限定公開
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Availability of remote photoduplication service: 可
Periodical Title (URI): http://id.nii.ac.jp/1392/00000191/
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