Comparison of the characteristics of mesenchymal stem-like cells derived by integration-free induced pluripotent stem cells in different single-cell culture media under feeder-free conditions

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Comparison of the characteristics of mesenchymal stem-like cells derived by integration-free induced pluripotent stem cells in different single-cell culture media under feeder-free conditions

Persistent ID (NDL): info:ndljp/pid/12071552

Material type: 博士論文

Author: 上田, 衛

Publisher: -

Publication date: 2019-03-08

Material Format: Digital

Capacity, size, etc.: -

Name of awarding university/degree: 大阪歯科大学,博士（歯学）

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Note (General)：: Generating mesenchymal stem-like cells (MSLCs) from induced pluripotent stem cells (iPSCs) can be a practical method for obtaining the sufficient cell...

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Material Type: 博士論文
Title: Comparison of the characteristics of mesenchymal stem-like cells derived by integration-free induced pluripotent stem cells in different single-cell culture media under feeder-free conditions
Author/Editor: 上田, 衛
Author Heading: 上田, 衛
Publication Date: 2019-03-08
Publication Date (W3CDTF): 2019-03-08
Alternative Title: 異なるフィーダーフリーシングルセル培養液を用いたインテグレーションフリーiPS細胞から誘導した間葉系幹細胞様細胞の特性比較
Degree grantor/type: 大阪歯科大学
Date Granted: 2019-03-08
Date Granted (W3CDTF): 2019-03-08
Dissertation Number: 甲第835号
Degree Type: 博士（歯学）
Conferring No. (Dissertation): 甲第835号
Text Language Code: eng
Subject Heading: Mesenchymal stem-like cells
Feeder-free conditions
Induced pluripotent stem cells
Human skin
Target Audience: 一般
Note (General): Generating mesenchymal stem-like cells (MSLCs) from induced pluripotent stem cells (iPSCs) can be a practical method for obtaining the sufficient cells for autologous tissue engineering. Single-cell culturing in specific medium and non-feeder cells is an alternative and promising strategy to overcome problems of embryo culture; however, little is known about how different culture media affect the proliferation and differentiation of MSLCs. We first derived MSLCs from iPSCs with non-integrating episomal plasmid vectors (hereafter 409B2 cells) using three different cell culture media, including single-cell culture medium in feeder-free condition: mTeSR1, DEF-CS500, or StemFit AK02N. The morphology of all MSLCs was completely altered to a fibroblastic morphology after four passages. Surface antigens CD29, CD44, CD73, CD90, but not CD34 and CD45, were expressed in all passages. RUNX2 was expressed in MSLCs cultured in all three feeder-free media, while SOX9 and PPARγ were expressed in MSLCs cultured in only DEF-CS500. MSLCs derived from DEF-CS500, which is a single-cell culture medium, grew at a slightly faster rate than those cultured in other media and expressed early-stage genes for tri-lineage differentiation. Taken together, these findings provide valuable information for generating MSLCs using single-cell culture methods.
2018年度
Persistent ID (NDL): info:ndljp/pid/12071552
https://dl.ndl.go.jp/pid/12071552
Collection: 障害者向け資料
Collection (Materials For Handicapped People:1): テキストデータ
Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
https://dl.ndl.go.jp/collections/A00014
Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2022-02-06T04:33:19+09:00
Date Created (W3CDTF): 2019-05-17
Format (IMT): application/pdf
Access Restrictions: 国立国会図書館内限定公開
Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
Availability of remote photoduplication service: 可
Periodical Title (URI): http://id.nii.ac.jp/1392/00000188/
Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション
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