Alternative Title口腔粘膜疾患への 8-MOP 使用によるフォトダイナミックセラピーの検討
Note (General)Psoralen ultraviolet A (PUVA) therapy using the cytotoxicity of ultraviolet radiation combined with 8-methoxypsoralen (8-MOP) is performed in the field of dermatol-ogy. As there are many disorders common to the oral mucosa and skin, treat-ments for refractory disorders of the skim may also be applicable to oral mucosal disorders. We, therefore, performed phototoxicity tests using 8-MOP primarily on cultured cells from oral tissues.The cell lines used were the mouse Balb/3T3, rat osteosarcoma UMR-106, rat squamous cell carcinoma SCC-158, human oral squamous cell carcinoma HSC-2, and human tongue squamous cell carcinoma HSC-3 and HSC-4. 8-MOP was added to the culture medium in 2-dimensional culture and 3-dimensional cul-ture using porcine Type I collagen gel. The cells were irradiated with ultraviolet light at 375 nm using an LED irradiation system. The control group was not irradiated. After 24-hour static culture, the cell survival rate was determined by MTT assay.In 2-dimensional culture, strong phototoxicity tended to be observed in cells de-rived from tumors. In 3-dimensional culture, phototoxicity varied in strength among cell types, and it was milder than in 2-dimensional culture, suggesting insufficient transmission of ultraviolet light to cells through the collagen gel. In cultured cells derived from tumors, the effects of photoirradiation varied among cell lines. As collagen may have inhibited transmission of ultraviolet light, a method to more ef-ficiently deliver ultraviolet light to cells in deep areas of the body is needed.
2017年度
Collection (particular)国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
Date Accepted (W3CDTF)2022-02-06T04:33:19+09:00
Data Provider (Database)国立国会図書館 : 国立国会図書館デジタルコレクション