Effect of platelet-rich plasma on proliferation of human synovial cells

Persistent ID (NDL): info:ndljp/pid/12071629

Material type: 博士論文

Author: 矢谷, 真也

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Publication date: 2016-03-11

Material Format: Digital

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Name of awarding university/degree: 大阪歯科大学,博士（歯学）

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Note (General)：: Platelet-rich plasma (PRP) promotes early tissue healing and regeneration, and significantly induces proliferation of various cells. We investigated h...

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Material Type: 博士論文
Title: Effect of platelet-rich plasma on proliferation of human synovial cells
Author/Editor: 矢谷, 真也
Author Heading: 矢谷, 真也
Publication Date: 2016-03-11
Publication Date (W3CDTF): 2016-03-11
Alternative Title: ヒト滑膜細胞の増殖に及ぼす多血小板血漿の影響
Degree grantor/type: 大阪歯科大学
Date Granted: 2016-03-11
Date Granted (W3CDTF): 2016-03-11
Dissertation Number: 甲第781号
Degree Type: 博士（歯学）
Conferring No. (Dissertation): 甲第781号
Text Language Code: eng
Subject Heading: Platelet-rich plasma
Human synovial cells
Interleukins
Target Audience: 一般
Note (General): Platelet-rich plasma (PRP) promotes early tissue healing and regeneration, and significantly induces proliferation of various cells. We investigated how PRP affects the proliferation of human synovial cells, which is not well understood. Surgical specimens of synovium were digested and the liberated cells resuspended in Dulbecco’s modified Eagle’s medium (DMEM). PRP was prepared using the double spin method, and activated PRP (aPRP) was prepared with calcium chloride and autologous thrombin. Cell proliferation was examined using Cell Counting Kit-8 (Dojindo Molecular Technologies, Gaithersburg, MD, USA) and the protein concentrations of IL-6 and IL-8 in culture media were determined using the Artemis TR-FRET Microplate Reader for HTRF® (Cosmo Bio, Tokyo, Japan) with HTRF® reagents (Cisbio, Marcoule, France). We found that aPRP promoted proliferation of human synovial cells more than did PRP on day 5 of culture and that it promoted proliferation in a concentration-dependent manner. Proteins of IL-6 and IL-8 were up-regulated in culture media. 5% aPRP was the lowest relative value per cell among IL-1β, -6 and -8. Thus, we determined an optimal concentration of 5%.
2015
Persistent ID (NDL): info:ndljp/pid/12071629
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Collection: 障害者向け資料
Collection (Materials For Handicapped People:1): テキストデータ
Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
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Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2022-02-06T04:33:19+09:00
Date Created (W3CDTF): 2016-07-19
Format (IMT): application/pdf
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Periodical Title (URI): http://id.nii.ac.jp/1392/00000102/
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