The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells

• Material Type: 博士論文
• Title: The effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation and osteoblastic differentiation of human mesenchymal stem cells
• Author/Editor: ⽚⼭, 暢仁
• Author Heading: ⽚⼭, 暢仁
• Publication Date: 2015-03-06
• Publication Date (W3CDTF): 2015-03-06
• Alternative Title: ヒト間葉系幹細胞に対するエナメルマトリックス由来合成ペプチドの影響
• Degree grantor/type: 大阪歯科大学
• Date Granted: 2015-03-06
• Date Granted (W3CDTF): 2015-03-06
• Dissertation Number: 甲第744号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第744号
• Text Language Code: eng
• Subject Heading: Alkaline Phosphatase; Cell Differentiation; Cell Proliferation; Cells, Cultured; Collagen Type I; Dental Enamel; Flavonoids; Humans; Mesenchymal Stromal Cells; Oligopeptides; Osteocalcin
• Target Audience: 一般
• Note (General): Enamel matrix derivative (EMD) is widely used in periodontal tissue regeneration therapy. However, because the bioactivity of EMD varies from batch to batch, and the use of a synthetic peptide could avoid use from an animal source, a completely synthetic peptide (SP) containing the active component of EMD would be useful. In this study an oligopeptide synthesized derived from EMD was evaluated for whether it contributes to periodontal tissue regeneration. We investigated the effects of the SP on cell proliferation and osteoblast differentiation of human mesenchymal stem cells (MSCs), which are involved in tissue regeneration. MSCs were treated with SP (0 to 1000 ng/mL), to determine the optimal concentration. We examined the effects of SP on cell proliferation and osteoblastic differentiation indicators such as alkaline phosphatase activity, the production of procollagen type 1 C-peptide and osteocalcin, and on mineralization. Additionally, we investigated the role of extracellular signal-related kinases (ERK) in cell proliferation and osteoblastic differentiation induced by SP. Our results suggest that SP promotes these processes in human MSCs, and that ERK inhibitors suppress these effects. In conclusion, SP promotes cell proliferation and osteoblastic differentiation of human MSCs, probably through the ERK pathway.; 2014年度
• Persistent ID (NDL): info:ndljp/pid/12071664; https://dl.ndl.go.jp/pid/12071664
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2022-02-06T04:33:19+09:00
• Date Created (W3CDTF): 2016-06-20
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
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• Periodical Title (URI): http://id.nii.ac.jp/1392/00000006/
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp