Bile duct reconstruction using scaffold-free tubular constructs created by Bio-3D printer

• Material Type: 博士論文
• ISSN: 2352-3204
• Title: Bile duct reconstruction using scaffold-free tubular constructs created by Bio-3D printer
• Volume: 16
• Author/Editor: 濵田, 隆志
• Author Heading: 濵田, 隆志
• Publication, Distribution, etc.: 日本再生医療学会
• Publication Date: 2021-09-01
• Publication Date (W3CDTF): 2021-09-01
• Alternative Title: Bio-3Dプリンターで作製したscaffold freeの管状構造体を用いた胆管再建
• Periodical title: Regenerative Therapy
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2021-09-01
• Date Granted (W3CDTF): 2021-09-01
• Dissertation Number: 甲医歯薬第1360号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲医歯薬第1360号
• Text Language Code: eng
• Subject Heading: Artificial bile duct; Bio-3D printer; Reconstruction; Scaffold-free tubular construct
• Target Audience: 一般
• Note (General): Introduction: Biliary strictures after bile duct injury or duct-to-duct biliary reconstruction are serious complications that markedly reduce patients’ quality of life because their treatment involves periodic stent replacements. This study aimed to create a scaffold-free tubular construct as an interposition graft to treat biliary complications.Methods: Scaffold-free tubular constructs of allogeneic pig fibroblasts, that is, fibroblast tubes, were created using a Bio-3D Printer and implanted into pigs as interposition grafts for duct-to-duct biliary reconstruction.Results: Although the fibroblast tube was weaker than the native bile duct, it was sufficiently strong to enable suturing. The pigs' serum hepatobiliary enzyme levels remained stable during the experimental period. Micro-computed tomography showed no biliary strictures, no biliary leakages, and no intrahepatic bile duct dilations. The tubular structure was retained in all resected specimens, and the fibroblasts persisted at the graft sites. Immunohistochemical analyses revealed angiogenesis in the fibroblast tube and absence of extensions of the biliary epithelium into the fibroblast tube's lumen.Conclusions: This study's findings demonstrated successful reconstruction of the extrahepatic bile duct with a scaffold-free tubular construct created from pig fibroblasts using a novel Bio-3D Printer. This construct could provide a novel regenerative treatment for patients with hepatobiliary diseases.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1360号 学位授与年月日:令和3年9月1日; Author: Takashi Hamada, Anna Nakamura, Akihiko Soyama, Yusuke Sakai, Takayuki Miyoshi, Shun Yamaguchi, Masaaki Hidaka, Takanobu Hara, Tota Kugiyama, Mitsuhisa Takatsuki, Akihide Kamiya, Koichi Nakayama, Susumu Eguchi; Citation: Regenerative Therapy, 16, pp.81-89; 2021; identifier:Nagasaki University (長崎大学), 博士(医学) (2021-09-01); http://hdl.handle.net/10069/00040946
• DOI: info:doi/10.1016/j.reth.2021.02.001; https://doi.org/info:doi/10.1016/j.reth.2021.02.001
• Persistent ID (NDL): info:ndljp/pid/12154370; https://dl.ndl.go.jp/pid/12154370
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2022-03-06T04:54:00+09:00
• Date Created (W3CDTF): 2022-02-10
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Related Material (URI): http://hdl.handle.net/10069/00040946
• Periodical Title (URI): http://hdl.handle.net/10069/00041248
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp