A Combination of GM-CSF and Released Factors from Gamma-Irradiated Tumor Cells Enhances the Differentiation of Macrophages from Bone Marrow Cells and Their Antigen-Presenting Function and Polarization to Type 1 7

• Material Type: 博士論文
• ISSN: 2305-6320
• Title: A Combination of GM-CSF and Released Factors from Gamma-Irradiated Tumor Cells Enhances the Differentiation of Macrophages from Bone Marrow Cells and Their Antigen-Presenting Function and Polarization to Type 1
• Volume: 8 7
• Author/Editor: Chen, Lichao
• Author Heading: Chen, Lichao
• Publication, Distribution, etc.: MDPI
• Publication Date: 2021-09-17; 8 7
• Publication Date (W3CDTF): 2021-09-17
• Alternative Title: GM-CSFとガンマ線照射腫瘍細胞の放出因子の組み合わせによる骨髄細胞からのマクロファージ分化と、それらの抗原提示機能および1型への極性化の促進
• Periodical title: Medicines
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2021-09-17
• Date Granted (W3CDTF): 2021-09-17
• Dissertation Number: 甲医歯薬第1359号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲医歯薬第1359号
• Text Language Code: eng
• Subject Heading: GM-CSF; macrophage; radiotherapy
• Target Audience: 一般
• Note (General): Granulocyte-macrophage colony-stimulating factor (GM-CSF) promotes dendritic cell differentiation from precursors, and consequently, enhances the antigen presentation process and adaptive immune responses. With such functions, GM-CSF has been used as immunotherapy in combination with radiotherapy for cancer treatment to augment the survival and activity of immune cells. However, an immune-suppressive tumor microenvironment may cause anergy of T cells. It has also been reported that GM-CSF contributes to the development of myeloid-derived suppressor cells from the precursors. In this study, to analyze the combined effect of GM-CSF and released factors from cancer cells after gamma-ray irradiation on bone marrow cell differentiation and dynamics, we established an in vitro culture system using mouse bone marrow cells, GM-CSF, and conditioned medium from gamma ray irradiated mouse melanoma B16 cells at 24 Gy. We analyzed the gene expression changes of the bone marrow-derived cells on day 6. The results showed that GM-CSF dose-dependently enhanced the differentiation of macrophages from bone marrow cells, their antigenpresenting function and polarization to type I. The results implied the induced macrophages from the bone marrow may potentially contribute to tumor immune responses in a systemic manner when GM-CSF is boosted during photon-beam radiation therapy.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1359号 学位授与年月日:令和3年9月17日; Author: Lichao Chen, Shoji Imamichi, Ying Tong, Yuka Sasaki, Takae Onodera, Satoshi Nakamura, Hiroshi Igaki, Jun Itami, Mitsuko Masutani; Citation: Medicines, 8(7), art. no. 35; 2021; identifier:Nagasaki University (長崎大学), 博士(医学) (2021-09-17)
• DOI: info:doi/10.3390/medicines8070035; https://doi.org/info:doi/10.3390/medicines8070035
• Persistent ID (NDL): info:ndljp/pid/12154371; https://dl.ndl.go.jp/pid/12154371
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2022-03-06T04:54:00+09:00
• Date Created (W3CDTF): 2022-02-10
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Related Material (URI): http://hdl.handle.net/10069/00040946
• Periodical Title (URI): http://hdl.handle.net/10069/00041247
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp