Aggregatibacter actinomycetemcomitans induces detachment and death of human gingival epithelial cells and fibroblasts via elastase release following leukotoxin-dependent neutrophil lysis

• Material Type: 博士論文
• Title: Aggregatibacter actinomycetemcomitans induces detachment and death of human gingival epithelial cells and fibroblasts via elastase release following leukotoxin-dependent neutrophil lysis
• Author/Editor: Hiyoshi, Takumi
• Author Heading: Hiyoshi, Takumi
• Publication Date: 2021-03-23
• Publication Date (W3CDTF): 2021-03-23
• Alternative Title: Aggregatibacter actinomycetemcomitansはロイコトキシンにより好中球を溶解し，エラスターゼを漏出させることで，ヒト歯肉上皮細胞とヒト歯肉線維芽細胞に細胞剥離および細胞死を誘導する
• Degree grantor/type: 新潟大学
• Date Granted: 2021-03-23
• Date Granted (W3CDTF): 2021-03-23
• Dissertation Number: 甲第4873号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第4873号
• Text Language Code: eng
• Alias of Author: 日吉, 巧
• Subject Heading: Aggregatibacter actinomycetemcomitans; leukotoxin; neutrophil elastase; periodontitis
• Target Audience: 一般
• Note (General): Aggregatibacter actinomycetemcomitans is considered to be associated with periodontitis. Leukotoxin (LtxA), which destroys leukocytes in humans, is one of this bacterium's major virulence factors. Amounts of neutrophil elastase (NE), which is normally localized in the cytoplasm of neutrophils, are reportedly increased in the saliva of patients with periodontitis. However, the mechanism by which NE is released from human neutrophils and the role of NE in periodontitis is unclear. In the present study, it was hypothesized that LtxA induces NE release from human neutrophils, which subsequently causes the breakdown of periodontal tissues. LtxA-treatment did not induce significant cytotoxicity against human gingival epithelial cells (HGECs) or human gingival fibroblasts (HGFs). However, it did induce significant cytotoxicity against human neutrophils, leading to NE release. Furthermore, NE and the supernatant from LtxA-treated human neutrophils induced detachment and death of HGECs and HGFs, these effects being inhibited by administration of an NE inhibitor, sivelestat. The present results suggest that LtxA mediates human neutrophil lysis and induces the subsequent release of NE, which eventually results in detachment and death of HGECs and HGFs. Thus, LtxA-induced release of NE could cause breakdown of periodontal tissue and thereby exacerbate periodontitis.; Microbiology and Immunology. 2019, 63(3-4), 100-110.; 新大院博(歯)第481号
• Persistent ID (NDL): info:ndljp/pid/12263511; https://dl.ndl.go.jp/pid/12263511
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2022-05-09T11:57:37+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10191/0002000369; https://niigata-u.repo.nii.ac.jp/records/2000369
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp