A Simple and High-Throughput ELISA-Based Neutralization Assay for the Determination of Anti-Flavivirus Neutralizing Antibodies

• Material Type: 博士論文
• ISSN: 2076-393X
• Title: A Simple and High-Throughput ELISA-Based Neutralization Assay for the Determination of Anti-Flavivirus Neutralizing Antibodies
• Volume: 8 2
• Author/Editor: Balingit, Jean Claude Palma
• Author Heading: Balingit, Jean Claude Palma
• Publication, Distribution, etc.: MDPI
• Publication Date: 2022-03-18
• Publication Date (W3CDTF): 2022-03-18
• Alternative Title: ELISA法を基盤とした簡便でハイスループットなフラビウイルス中和抗体測定法の開発
• Periodical title: Vaccines
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2022-03-18
• Date Granted (W3CDTF): 2022-03-18
• Dissertation Number: 甲医歯薬第1437号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲医歯薬第1437号
• Text Language Code: eng
• Subject Heading: ELISA; microneutralization test; flavivirus; neutralizing antibodies; dengue; Zika
• Target Audience: 一般
• Note (General): Mosquito-borne flavivirus infections, including dengue virus and Zika virus, are major public health threats globally. While the plaque reduction neutralization test (PRNT) is considered the gold standard for determining neutralizing antibody levels to flaviviruses, the assay is time-consuming and laborious. This study, therefore, aimed to develop an enzyme-linked immunosorbent assay (ELISA)-based microneutralization test (EMNT) for the detection of neutralizing antibodies to mosquito-borne flaviviruses. The inhibition of viral growth due to neutralizing antibodies was determined colorimetrically by using EMNT. Given the significance of Fcγ-receptors (FcγR) in antibody-mediated neutralization and antibody-dependent enhancement (ADE) of flavivirus infection, non-FcγR and FcγR-expressing cell lines were used in the EMNT to allow the detection of the sum of neutralizing and immune-enhancing antibody activity as the neutralizing titer. Using anti-flavivirus monoclonal antibodies and clinical samples, the utility of EMNT was evaluated by comparing the end-point titers of the EMNT and the PRNT. The correlation between EMNT and PRNT titers was strong, indicating that EMNT was robust and reproducible. The new EMNT assay combines the biological functional assessment of virus neutralization activity and the technical advantages of ELISA and, is simple, reliable, practical, and could be automated for high-throughput implementation in flavivirus surveillance studies and vaccine trials.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1437号 学位授与年月日:令和4年3月18日; Author: Jean Claude Balingit, Minh Huong Phu Ly, Mami Matsuda, Ryosuke Suzuki, Futoshi Hasebe, Kouichi Morita and Meng Ling Moi; Citation: Vaccines, 8(2), art.no. 297; 2020; identifier:Nagasaki University (長崎大学), 博士(医学) (2022-03-18); http://hdl.handle.net/10069/00041440
• DOI: info:doi/10.3390/vaccines8020297; https://doi.org/info:doi/10.3390/vaccines8020297
• Persistent ID (NDL): info:ndljp/pid/12296682; https://dl.ndl.go.jp/pid/12296682
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2022-06-05T18:01:14+09:00
• Date Created (W3CDTF): 2022-05-26
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Related Material (URI): http://hdl.handle.net/10069/00041440
• Periodical Title (URI): http://hdl.handle.net/10069/00041555
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp