Intrathymic Plasmablasts Are Affected in Patients With Myasthenia Gravis With Active Disease

• Material Type: 博士論文
• ISSN: 2332-7812
• Title: Intrathymic Plasmablasts Are Affected in Patients With Myasthenia Gravis With Active Disease
• Volume: 8 6
• Author/Editor: Yamamoto, Yohei; Matsui, Naoko; Uzawa, Akiyuki; Ozawa, Yukiko; Kanai, Tetsuya; Oda, Fumiko; Kondo, Hiroyuki; Ohigashi, Izumi; Takizawa, Hiromitsu; Kondo, Kazuya; Sugano, Mikio; Kitaichi, Takashi; Hata, Hiroki; Kaji, Ryuji; Kuwabara, Satoshi; Yamamura, Takashi; Izumi, Yuishin
• Author Heading: Yamamoto, Yohei; Matsui, Naoko; Uzawa, Akiyuki; Ozawa, Yukiko; Kanai, Tetsuya; Oda, Fumiko; Kondo, Hiroyuki; Ohigashi, Izumi; Takizawa, Hiromitsu; Kondo, Kazuya; Sugano, Mikio; Kitaichi, Takashi; Hata, Hiroki; Kaji, Ryuji; Kuwabara, Satoshi; Yamamura, Takashi; Izumi, Yuishin
• Publication, Distribution, etc.: Wolters Kluwer|American Academy of Neurology
• Publication Date: 2021-09-24
• Publication Date (W3CDTF): 2021-09-24
• Alternative Title: 疾患活動性の高い重症筋無力症患者の胸腺ではプラズマブラストが増加している
• Periodical title: Neurology : Neuroimmunology & Neuroinflammation
• Degree grantor/type: 徳島大学
• Date Granted: 2022-03-04
• Date Granted (W3CDTF): 2022-03-04
• Dissertation Number: 甲第3581号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第3581号
• Text Language Code: eng
• Subject Heading: Myasthenia Gravis; Thymectomy; Plasmablast; B cell; Tfh cell
• Target Audience: 一般
• Note (General): Background and ObjectivesTo investigate intrathymic B lymphopoiesis in patients with myasthenia gravis (MG) and explore thymus pathology associated with clinical impact.MethodsThymic lymphocytes from 15 young patients without MG, 22 adult patients without MG, 14 patients with MG without thymoma, and 11 patients with MG with thymoma were subjected to flow cytometry analysis of T follicular helper (Tfh), naive B, memory B, plasmablasts, CD19+B220high thymic B cells, B-cell activating factor receptor, and C-X-C chemokine receptor 5 (CXCR5). Peripheral blood mononuclear cells of 16 healthy subjects and 21 untreated patients with MG were also analyzed. Immunologic values were compared, and correlations between relevant values and clinical parameters were evaluated.ResultsThe frequencies of circulating and intrathymic plasmablasts were significantly higher in patients with MG than controls. On the other hand, the frequency of CD19+B220high thymic B cells was not increased in MG thymus. We observed a significant increase in CXCR5 expression on plasmablasts in MG thymus and an increased frequency of intrathymic plasmablasts that was correlated with preoperative disease activity. The frequency of intrathymic Tfh cells was significantly lower in patients who received immunosuppressive (IS) therapy than those without IS therapy. However, there was no significant difference in the frequency of intrathymic plasmablasts irrespective of IS therapy.DiscussionOur findings confirmed a correlation between increased frequency of intrathymic plasmablasts and disease activity before thymectomy. We postulate that activated intrathymic plasmablasts endow pathogenic capacity in MG.
• DOI: info:doi/10.1212/NXI.0000000000001087; https://doi.org/info:doi/10.1212/NXI.0000000000001087
• Persistent ID (NDL): info:ndljp/pid/12296804; https://dl.ndl.go.jp/pid/12296804
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• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2022-06-05T18:01:14+09:00
• Format (IMT): application/pdf
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• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/116884
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