Enhancement of Bone-Forming Ability on Beta-Tricalcium Phosphate by Modulating Cellular Senescence Mechanisms Using Senolytics

• Material Type: 博士論文
• Title: Enhancement of Bone-Forming Ability on Beta-Tricalcium Phosphate by Modulating Cellular Senescence Mechanisms Using Senolytics
• Author/Editor: 王, 欣琛
• Author Heading: 王, 欣琛
• Publication Date: 2022-03-04
• Publication Date (W3CDTF): 2022-03-04
• Alternative Title: セノリティクス薬を用いた細胞老化機序の制御によるβ型リン酸三カルシウムの骨形成能の向上
• Degree grantor/type: 大阪歯科大学
• Date Granted: 2022-03-04
• Date Granted (W3CDTF): 2022-03-04
• Dissertation Number: 甲第923号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第923号
• Text Language Code: eng
• Subject Heading: Cellular senescence｜Beta-tricalcium phosphate｜ Bone formation｜Senolytics
• Target Audience: 一般
• Note (General): Various stresses latently induce cellular senescence that occasionally deteriorates the functioning of surrounding tissues. Nevertheless, little is known about the appearance and function of senescent cells, caused by the implantation of beta-tricalcium phosphate (β-TCP)—used widely in dentistry and orthopedics for treating bone diseases. In this study, two varying sizes of β-TCP granules (<300 µm and 300–500 µm) were implanted, and using histological and immunofluorescent staining, appearances of senescent-like cells in critical-sized bone defects in the calvaria of Sprague Dawley rats were evaluated. Parallelly, bone formation in defects was investigated with or without the oral administration of senolytics (a cocktail of dasatinib and quercetin). A week after the implantation, the number of senescence-associated beta-galactosidase, p21-, p19-, and tartrate-resistant acid phosphatase-positive cells increased and then decreased upon administrating senolytics. This administration of senolytics also attenuated 4-hydroxy-2-nonenal staining, representing reactive oxygen species. Combining senolytic administration with β-TCP implantation significantly enhanced the bone formation in defects as revealed by micro-computed tomography analysis and hematoxylin-eosin staining. This study demonstrates that β-TCP granules latently induce senescent-like cells, and senolytic administration may improve the bone-forming ability of β-TCP by inhibiting senescence-associated mechanisms.; 2021年度
• Persistent ID (NDL): info:ndljp/pid/12296873; https://dl.ndl.go.jp/pid/12296873
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2022-06-05T18:01:14+09:00
• Date Created (W3CDTF): 2022-05-13
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://id.nii.ac.jp/1392/00000300/
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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2023-09-07 再収集