Additional effects of simultaneous treatment with C14-Cblin and celastrol on the clinorotation-induced rat L6 myotube atrophy

• Material Type: 博士論文
• ISSN: 1349-6867
• Title: Additional effects of simultaneous treatment with C14-Cblin and celastrol on the clinorotation-induced rat L6 myotube atrophy
• Volume: 69 1-2
• Author/Editor: Kitahata, Kanako; Uchida, Takayuki; Taniguchi, Runa; Kato, Ayano; Sugiura, Kosuke; Sakakibara, Iori; Oarada, Motoko; Fukawa, Tomoya; Junsoo, Park; Inho, Choi; Nikawa, Takeshi
• Author Heading: Kitahata, Kanako; Uchida, Takayuki; Taniguchi, Runa; Kato, Ayano; Sugiura, Kosuke; Sakakibara, Iori; Oarada, Motoko; Fukawa, Tomoya; Junsoo, Park; Inho, Choi; Nikawa, Takeshi
• Publication, Distribution, etc.: Tokushima University Faculty of Medicine
• Publication Date: 2022-02
• Publication Date (W3CDTF): 2022-02
• Alternative Title: 3D-clinorotationによるラットL6筋管萎縮に対するC14-Cblin及びcelastrolの同時投与による相加効果; Effect of Cblin and celastrol on muscle atrophy
• Periodical title: The Journal of Medical Investigation
• Degree grantor/type: 徳島大学
• Date Granted: 2022-03-10
• Date Granted (W3CDTF): 2022-03-10
• Dissertation Number: 甲第3584号
• Degree Type: 博士（栄養学）
• Conferring No. (Dissertation): 甲第3584号
• Text Language Code: eng
• Subject Heading: C14-Cblin; celastrol; Cbl-b; 筋萎縮; 酸化ストレス; myotube atrophy; muscle atrophy-associated ubiquitin ligases; oxidative stress
• Target Audience: 一般
• Note (General): Two novel reagents, N-myristoylated Cbl-b inhibitory peptide (C14-Cblin) and celastrol, a quinone methide triterpene, are reported to be effective in preventing myotube atrophy. The combined effects of C14-Cblin and celastrol on rat L6 myotubes atrophy induced by 3D-clinorotation, a simulated microgravity model, was investigated in the present study. We first examined their effects on expression in atrogenes. Increase in MAFbx1/atrogin-1 and MuRF-1 by 3D-clinorotation was significantly suppressed by treatment with C14-Cblin or celastrol, but there was no additive effect of simultaneous treatment. However, celastrol significantly suppressed the upregulation of Cbl-b and HSP70 by 3D-clinorotation. Whereas 3D-clinorotation decreased the protein level of IRS-1 in L6 myotubes, C14-Cblin and celastrol inhibited the degradation of IRS-1. C14-Cblin and celastrol promoted the phosphorylation of FOXO3a even in microgravity condition. Simultaneous administration of C14-Cblin and celastrol had shown little additive effect in reversing the impairment of IGF-1 signaling by 3D-clinorotation. While 3D-clinorotation-induced marked oxidative stress in L6 myotubes, celastrol suppressed 3D-clinorotation-induced ROS production. Finally, the C14-Cblin and celastrol-treated groups were inhibited decrease in L6 myotube diameter and increased the protein content of slow-twitch MyHC cultured under 3D-clinorotation. The simultaneous treatment of C14-Cblin and celastrol additively prevented 3D-clinorotation-induced myotube atrophy than single treatment.
• DOI: info:doi/10.2152/jmi.69.127; https://doi.org/info:doi/10.2152/jmi.69.127
• Persistent ID (NDL): info:ndljp/pid/12297067; https://dl.ndl.go.jp/pid/12297067
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2022-06-05T18:01:14+09:00
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
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• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/116937
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp