Expression and functional maintenance of volume-regulated anion channels in myometrial smooth muscles of pregnant mice.

• Material Type: 博士論文
• ISSN: 1881-7122
• Title: Expression and functional maintenance of volume-regulated anion channels in myometrial smooth muscles of pregnant mice.
• Volume: 71 2
• Author/Editor: 山田, 一貴; YAMADA, Kazutaka
• Author Heading: 山田, 一貴; YAMADA, Kazutaka
• Publication, Distribution, etc.: Japanese Association for Laboratory Animal Science
• Publication Date: 2022-05-20
• Publication Date (W3CDTF): 2022-05-20
• Periodical title: Experimental animals
• Degree grantor/type: 滋賀医科大学
• Date Granted: 2022-03-10
• Date Granted (W3CDTF): 2022-03-10
• Dissertation Number: 甲第925号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第925号
• Text Language Code: eng
• Subject Heading: leucine-rich repeat containing 8A; myometrium; pregnancy; swelling-induced Cl–current; volume-regulated anion channel
• Target Audience: 一般
• Note (General): pdf; Pregnancy causes changes in the uterus, such as increased cell volume and altered water content. However, the mechanisms that protect the structure and maintain the function of uterine smooth muscle cells against these changes during pregnancy have not been clarified. This study focused on the volume-regulated anion channel (VRAC), which opens with cell swelling under low osmotic pressure and releases Cl– ions and various organic osmolytes to resist cell swelling and regulates a wide range of biological processes such as cell death. In this study, myometrial smooth muscle (MSM) tissues and cells (MSMCs) were collected from non-pregnant and pregnant mice. Using western blotting and immunocytochemistry, leucine-rich repeat containing protein 8A (LRRC8A), an essential membrane protein that constitutes part of the VRAC, was determined to be diffused throughout MSMCs including in the cell membrane. Patch-clamp experiments were performed to investigate the electrophysiology of swelling-induced Cl– currents (ICl, swell) mediated by the VRAC. No significant changes between non-pregnancy and pregnancy groups were observed in either the expression density of LRRC8A or the current density of ICl, swell, however the presence of LRRC8A on the cell membrane was significantly increased in the third trimester of pregnancy compared to the non-pregnancy. This study suggests that the VRAC may play a role, such as maintaining cellular homeostasis in the pregnant MSM.; Thesis or Dissertation; 令和3年度; 10.1538/expanim.21-0111; 博士論文要旨
• DOI: info:doi/10.1538/expanim.21-0111; https://doi.org/info:doi/10.1538/expanim.21-0111
• Persistent ID (NDL): info:ndljp/pid/12304055; https://dl.ndl.go.jp/pid/12304055
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2022-07-05T02:30:21+09:00
• Date Created (W3CDTF): 2022-06-14
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Original Material (URI): http://hdl.handle.net/10422/00013327
• Periodical Title (URI): http://hdl.handle.net/10422/00013326; https://shiga-med.repo.nii.ac.jp/records/4240
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp