Febuxostat treatment attenuates oxidative stress and inflammation due to ischemia-reperfusion injury through the necrotic pathway in skin flap of animal model

• Material Type: 博士論文
• Title: Febuxostat treatment attenuates oxidative stress and inflammation due to ischemia-reperfusion injury through the necrotic pathway in skin flap of animal model
• Author/Editor: Odake, Kazuya
• Author Heading: Odake, Kazuya
• Publication, Distribution, etc.: 三重大学
• Publication Date: 2022-03-25
• Publication Date (W3CDTF): 2022-03-25
• Degree grantor/type: 三重大学
• Date Granted: 2022-03-25
• Date Granted (W3CDTF): 2022-03-25
• Dissertation Number: 甲医学第2116号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲医学第2116号
• Text Language Code: eng
• Alias of Author: オダケ, カズヤ; 小嶽, 和也
• Subject Heading: Ischemia/reperfusion injury; Febuxostat; Skin flap; Oxidative stress; Inflammation; Necrosis
• Target Audience: 一般
• Note (General): application/pdf; Background: Ischemia-reperfusion (I/R) injury is a major contributor to skin flap necrosis, which is a serious complication of reconstructive surgery. The purpose of this study was to evaluate the protective effect of treatment with febuxostat, a selective xanthine oxidase inhibitor, on I/R injury in the skin flap of an animal (rat) model. Methods: Superficial epigastric flaps were raised in Sprague-Dawley rats and subjected to ischemia for 3h. Febuxostat at a dose of 10 mg/kg/day was administered to rats in drinking water from 1 week before the surgery (Feb group). Control animals received no drugs (Con group). The mean ratio of flap survival and contraction was evaluated and compared between animals with and without administration of febuxostat on day 5 after the surgery. In addition, infiltration by polymorphonuclear leukocytes and muscles of the panniculus carnosus in the flap were histologically evaluated using hematoxylin-eosin staining. Furthermore, xanthine oxidase activity, ATP levels, superoxide dismutase activity, and expression of 8-hydroxy-2’-deoxyguanosine (8-OHdG), tumor necrosis factor-α, and interleukin-1β were quantitatively assessed in the skin flap 24 h after the surgery.Results: In the Feb group, the survival and contraction rates at the 5 d timepoint post-surgery were significantly higher and lower than those in the Con group, respectively. Histological analysis showed significant reduction in polymorphonuclear leukocyte infiltration and muscle injury scores due to I/R injury in the Feb group. The expression of 8-OHdG was also significantly inhibited in animals administered febuxostat. Biochemical analysis showed a significant reduction in xanthine oxidase activity and significant increases in ATP levels and superoxide dismutase activity in the Feb group. Furthermore, the expression of interleukin-1β was significantly lower in the Feb group than in the Con group.Conclusion: Febuxostat, which is clinically used for the treatment of hyperuricemia, was effective against necrosis of the skin flap via inhibition of oxidative stress and inflammation caused by I/R injury.; 本文/Department of Orthopaedic Surgery, Graduate School of Medicine, Mie University, Japan; 9p
• DOI: info:doi/10.1016/j.freeradbiomed.2021.10.033; https://doi.org/info:doi/10.1016/j.freeradbiomed.2021.10.033
• Persistent ID (NDL): info:ndljp/pid/12313672; https://dl.ndl.go.jp/pid/12313672
• Collection: 障害者向け資料
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• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2022-08-08T06:02:54+09:00
• Date Created (W3CDTF): 2022-07-20
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
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• Periodical Title (URI): http://hdl.handle.net/10076/00020582; https://mie-u.repo.nii.ac.jp/records/14964
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp