Contribution of long-chain fatty acid to induction of myeloid-derived suppressor cell (MDSC)-like cells - induction of MDSC by lipid vesicles (liposome) （長鎖脂肪酸による骨髄由来免疫抑制細胞様細胞誘導への関与―脂質小胞（リポソーム）によるMDSCの誘導） 6

• Material Type: 博士論文
• ISSN: 0892-3973
• Title: Contribution of long-chain fatty acid to induction of myeloid-derived suppressor cell (MDSC)-like cells - induction of MDSC by lipid vesicles (liposome) （長鎖脂肪酸による骨髄由来免疫抑制細胞様細胞誘導への関与―脂質小胞（リポソーム）によるMDSCの誘導）
• Volume: 42 6
• Author/Editor: 吉田, 陽一郎
• Author Heading: 吉田, 陽一郎
• Publication Date: 2020-12
• Publication Date (W3CDTF): 2020-12
• Periodical title: Immunopharmacol Immunotoxicol
• Degree grantor/type: 旭川医科大学
• Date Granted: 2021-09-30
• Date Granted (W3CDTF): 2021-09-30
• Dissertation Number: 乙第491号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 乙第491号
• Text Language Code: eng
• Subject Heading: B7-H3; NFκB; iNOS; macrophages; microvesicle
• Target Audience: 一般
• Note (General): type:application/pdf; Context: Effects of liposomal particles on immune function have not been adequately investigated. Earlier reports indicate that intravenous injection of rats with pegylated liposomes comprising chemically defined specific lipids produces myeloid derived suppressor-cell (MDSC)-like cells in the spleen.Objectives: After liposome injection, we sought a cell surface marker expressed specifically on splenic macrophages. Then we assessed the immunosuppressive activity of macrophages positive for the marker. Furthermore, we investigated whether immunosuppression induction is an immunopharmacological action specific to this pegylated liposome, or not.Materials and methods: After using a microarray system to screen genes enhanced by this liposome, we evaluated cell surface expression of gene products using flow cytometry. Liposomes of several kinds, each comprising one type of phospholipid, were prepared and evaluated for their ability to induce T-cell suppression.Results: Microarray analysis indicated enhanced B7-H3 expression. Flow cytometry revealed that the B7-H3 molecule was expressed on splenic macrophages after liposome injection. B7-H3+ macrophages were positive for iNOS. Removing B7-H3+ cells restored T-cell proliferation. Similarly to this liposome, various liposomes with different long chain fatty acids induced T-cell suppression when accumulated in the spleen.Conclusions: Immunosuppressive cells induced by this pegylated liposome closely resemble MDSCs, especially B7-H3+ MDSCs. Immunosuppression induction is not a phenomenon specific to this liposome. Accumulation of long chain fatty acid in macrophages by internalization of liposomal nanoparticles might be related to macrophage acquisition of immunosuppressive activity in vivo.; identifier:PMID: 33070657
• DOI: info:doi/10.1080/08923973.2020.1837866; https://doi.org/info:doi/10.1080/08923973.2020.1837866
• Persistent ID (NDL): info:ndljp/pid/12314265; https://dl.ndl.go.jp/pid/12314265
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• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2022-08-08T06:02:54+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
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• Periodical Title (URI): https://amcor.asahikawa-med.ac.jp/modules/xoonips/detail.php?id=20210930_O491
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp