Preparation of Artificial Red Blood Cells (Hemoglobin Vesicles) Using the Rotation-Revolution Mixer for High Encapsulation Efficiency.

• Material Type: 博士論文
• ISSN: 2373-9878
• Title: Preparation of Artificial Red Blood Cells (Hemoglobin Vesicles) Using the Rotation-Revolution Mixer for High Encapsulation Efficiency.
• Volume: 7 6
• Author/Editor: Kure, Tomoko; Sakai, Hiromi
• Author Heading: Kure, Tomoko; Sakai, Hiromi
• Publication, Distribution, etc.: American Chemical Society
• Publication Date: 2021-06; 7 6
• Publication Date (W3CDTF): 2021-06
• Alternative Title: 自転−公転ミキサーを用いた人工赤血球（ヘモグロビン ベシクル）の高いカプセル化効率のための調製法
• Periodical title: ACS biomaterials science and engineering
• Degree grantor/type: 奈良県立医科大学
• Date Granted: 2021-09-29
• Date Granted (W3CDTF): 2021-09-29
• Dissertation Number: 甲第797号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第797号
• Text Language Code: eng
• Subject Heading: liposomes; hemoglobin; blood substitutes; homogenization; planetary mixer
• Target Audience: 一般
• Note (General): type:Thesis; Hemoglobin vesicles (Hb-V) are artificial red blood cells encapsulating highly concentrated hemoglobin (Hb) in liposomes comprising phospholipids, cholesterol, negatively charged lipids, and polyethylene glycol (PEG)-conjugated phospholipids. Safety and efficacy of Hb-V as a transfusion alternative have been extensively studied. For this study, we prepared Hb-V using the kneading method with a rotation-revolution mixer as an alternative to the conventional extrusion method. We optimized the kneading operation parameters to obtain Hb-V with a high yield. Results show that the Hb encapsulation efficiency was increased dramatically up to 74.2%, which is higher than that of the extrusion method (20%) because the kneading method enabled mixing of a highly concentrated carbonylhemoglobin (HbCO) solution (40 g/dL) and a considerably large amount of powdered lipids in only 10 min. The high viscosity of the Hb-lipid mixture paste (ca. 103-105 cP) favorably induces frictional heat by kneading and increases the paste temperature (ca. 60 °C), which facilitates lipid dispersion and liposome formation. During the kneading operation using a thermostable HbCO solution, Hb denaturation was prevented. Hb-V prepared using this method showed no marked changes in particle sizes, Hb denaturation, or Hb leakage from liposomes during two years of long-term storage-stability tests. Collectively, these results demonstrate that the kneading method using a rotation-revolution mixer shows good potential as a new method to produce Hb-V.; 博士（医学）・甲第797号・令和3年9月29日; This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS biomaterials science and engineering, copyright © 2021 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsbiomaterials.1c00424; identifier:ACS biomaterials science and engineering Vol.7 No.6 p.2835-2844 (2021 Jun); identifier:23739878; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3946; identifier:ACS biomaterials science and engineering, 7(6): 2835-2844
• DOI: info:doi/10.1021/acsbiomaterials.1c00424; https://doi.org/info:doi/10.1021/acsbiomaterials.1c00424
• Persistent ID (NDL): info:ndljp/pid/12314499; https://dl.ndl.go.jp/pid/12314499
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2022-08-08T06:02:54+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10564/3946
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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