Probiotic-derived polyphosphate accelerates intestinal epithelia wound healing through inducing platelet-derived mediators （乳酸菌由来長鎖ポリリン酸は血小板活性化を介して腸管上皮の創傷治癒を促進する）

• Material Type: 博士論文
• ISSN: 0962-9351
• Title: Probiotic-derived polyphosphate accelerates intestinal epithelia wound healing through inducing platelet-derived mediators （乳酸菌由来長鎖ポリリン酸は血小板活性化を介して腸管上皮の創傷治癒を促進する）
• Volume: 2021
• Author/Editor: 磯崎, 翔太郎
• Author Heading: 磯崎, 翔太郎
• Publication Date: 2021-03
• Publication Date (W3CDTF): 2021-03
• Periodical title: Mediators of Inflammation
• Degree grantor/type: 旭川医科大学
• Date Granted: 2022-03-25
• Date Granted (W3CDTF): 2022-03-25
• Dissertation Number: 甲第576号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第576号
• Text Language Code: eng
• Target Audience: 一般
• Note (General): type:application/pdf; Inflammatory bowel disease (IBD), such as ulcerative colitis (UC) and Crohn's disease (CD), is an intractable intestinal inflammation associated with the disruption of the intestinal mucosa. We previously demonstrated that Lactobacillus brevis-derived long-chain polyphosphate (poly P) improved the intestinal barrier function by the upregulation of cell adhesion and relieved intestinal inflammation, thereby exerting a curing effect on colitis in vitro, in vivo, and in an investigator-initiated clinical study of UC. However, how poly P improves mucosal defects induced by intestinal inflammation has not been elucidated. In this study, we detected the accumulation of platelets in inflamed tissues induced by poly P in a dextran sulfate sodium- (DSS-) induced colitis mouse model. A light transmission aggregometry analysis and scanning electron microscopy showed that poly P promoted the platelet aggregation. An SRB assay and ki-67 staining showed that the supernatant of poly P-treated platelet-rich plasma (PRP) increased intestinal epithelial cell growth. A wound healing assay showed that the supernatant of poly P-treated PRP, but not poly P itself, accelerated wound healing. A Western blotting analysis indicated that mitogen-activated protein kinase activation was induced by the supernatant of poly P-treated human PRP in the epithelial cells and its wound healing effect was significantly decreased by the inhibition of ERK signaling. These data suggested that platelet-derived mediators induced by poly P improved intestinal inflammation through the promotion of epithelial cell growth by the activation of the ERK signaling pathway. The mechanism is a novel host-microbe interaction through mammalian platelet-derived mediators induced by bacterial molecules.; identifier:PMID:33859537
• DOI: info:doi/10.1155/2021/5582943; https://doi.org/info:doi/10.1155/2021/5582943
• Persistent ID (NDL): info:ndljp/pid/12319253; https://dl.ndl.go.jp/pid/12319253
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2022-09-05T13:46:15+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
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• Periodical Title (URI): https://amcor.asahikawa-med.ac.jp/modules/xoonips/detail.php?id=20220325_K576
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp