Extracellular matrix gene expression in human trabecular meshwork cells following mechanical fluid flow stimulation （ヒト線維柱帯細胞への水流機械刺激による細胞外マトリックス遺伝子発現に関する研究）

Persistent ID (NDL): info:ndljp/pid/12356117

Material type: 博士論文

Author: 吉田, 光一

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Publication date: 2022-03

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Name of awarding university/degree: 旭川医科大学,博士（医学）

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Note (General)：: type:application/pdfAim: To investigate changes in extracellular matrix (ECM) gene expression in human trabecular meshwork (HTM) cells in response to ...

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Material Type: 博士論文
ISSN: 2222-3959
Title: Extracellular matrix gene expression in human trabecular meshwork cells following mechanical fluid flow stimulation （ヒト線維柱帯細胞への水流機械刺激による細胞外マトリックス遺伝子発現に関する研究）
Volume: 15 3
Author/Editor: 吉田, 光一
Author Heading: 吉田, 光一
Publication Date: 2022-03
Publication Date (W3CDTF): 2022-03
Periodical title: International journal of ophthalmology
Degree grantor/type: 旭川医科大学
Date Granted: 2022-06-30
Date Granted (W3CDTF): 2022-06-30
Dissertation Number: 乙第494号
Degree Type: 博士（医学）
Conferring No. (Dissertation): 乙第494号
Text Language Code: eng
Subject Heading: aqueous humor
extracellular matrix
glaucoma
intraocular pressure
shear stress
trabecular meshwork
Target Audience: 一般
Note (General): type:application/pdf
Aim: To investigate changes in extracellular matrix (ECM) gene expression in human trabecular meshwork (HTM) cells in response to mechanical fluid flow stimulation.Methods: HTM cells were grown on a glass plate coated with 0.02% type I collagen (COL) and exposed to shear stress (0, 0.2, 1.0 dyne/cm2) for 12h. Changes in genes related to the ECM were evaluated by real-time reverse transcriptase-polymerase chain reaction. Phosphorylation of Smad2 protein was investigated by Western blotting.Results: After mechanical stimulation, COL type 4 alpha 2, COL type 6 alpha 1, and fibronectin-1 mRNA were significantly higher than the static control (P<0.05, <0.05, and <0.01, respectively). The metalloproteinase-2 and plasminogen activator inhibitor-1 mRNA were significantly higher than the static control (P<0.05 and <0.01, respectively), while the differences in the tissue inhibitors of metalloproteinases-2 mRNA were not significant. The phosphorylation of Smad2 levels was significantly higher compared to the static control cells.Conclusion: Changes in the expressions of genes associated ECM metabolism result in HTM cells after mechanical stimulation. The mechanical stimulation of the aqueous humor to the trabecular meshwork may promote ECM turnover and contribute to intraocular pressure homeostasis
identifier:PMID:35310043
DOI: info:doi/10.18240/ijo.2022.03.03
https://doi.org/info:doi/10.18240/ijo.2022.03.03
Persistent ID (NDL): info:ndljp/pid/12356117
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Collection (Materials For Handicapped People:1): テキストデータ
Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
https://dl.ndl.go.jp/collections/A00014
Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2022-10-11T11:55:44+09:00
Format (IMT): PDF
Access Restrictions: 国立国会図書館内限定公開
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Availability of remote photoduplication service: 可
Periodical Title (URI): https://amcor.asahikawa-med.ac.jp/modules/xoonips/detail.php?id=20220630_O494
Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション
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