Elevated cellular PpIX potentiates sonodynamic therapy in a mouse glioma stem cell-bearing glioma model by downregulating the Akt/NF-кB/MDR1 pathway

• Material Type: 博士論文
• ISSN: 2045-2322
• Title: Elevated cellular PpIX potentiates sonodynamic therapy in a mouse glioma stem cell-bearing glioma model by downregulating the Akt/NF-кB/MDR1 pathway
• Volume: 11
• Author/Editor: Shono, Kenji; Mizobuchi, Yoshifumi; Yamaguchi, Izumi; Nakajima, Kohei; Fujiwara, Yuri; Fujihara, Toshitaka; Kitazato, Keiko; Matsuzaki, Kazuhito; Uto, Yoshihiro; Sampetrean, Oltea; Saya, Hideyuki; Takagi, Yasushi
• Author Heading: Shono, Kenji; Mizobuchi, Yoshifumi; Yamaguchi, Izumi; Nakajima, Kohei; Fujiwara, Yuri; Fujihara, Toshitaka; Kitazato, Keiko; Matsuzaki, Kazuhito; Uto, Yoshihiro; Sampetrean, Oltea; Saya, Hideyuki; Takagi, Yasushi
• Publication, Distribution, etc.: Springer Nature
• Publication Date: 2021-07-23
• Publication Date (W3CDTF): 2021-07-23
• Alternative Title: Akt/NF-кB/MDR1経路の抑制による脳腫瘍細胞内のPpIXの上昇はグリオーマ幹細胞移植脳腫瘍モデルマウスの超音波力学療法の効果を増強する
• Periodical title: Scientific Reports
• Degree grantor/type: 徳島大学
• Date Granted: 2021-10-28
• Date Granted (W3CDTF): 2021-10-28
• Dissertation Number: 甲第3560号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第3560号
• Text Language Code: eng
• Subject Heading: Sonodynamic therapy; PpIX; stem cell; stem cell-bearing glioma model
• Target Audience: 一般
• Note (General): Glioblastoma (GBM) has high mortality rates because of extreme therapeutic resistance. During surgical resection for GBM, 5-aminolevulinic acid (5-ALA)-induced protoporphyrin IX (PpIX) fluorescence is conventionally applied to distinguish GBM. However, surgical intervention is insufficient for high invasive GBM. Sonodynamic therapy (SDT) combined with low-intensity ultrasonication (US) and PpIX, as a sonosensitizer, is an emerging and promising approach, although its efficacy is limited. Based on our previous study that down-regulation of multidrug resistant protein (MDR1) in GBM augmented the anti-tumor effects of chemotherapy, we hypothesized that elevation of cellular PpIX levels by down-regulation of MDR1 enhances anti-tumor effects by SDT. In high invasive progeny cells from mouse glioma stem cells (GSCs) and a GSC-bearing mouse glioma model, we assessed the anti-tumor effects of SDT with a COX-2 inhibitor, celecoxib. Down-regulation of MDR1 by celecoxib increased cellular PpIX levels, as well as valspodar, an MDR1 inhibitor, and augmented anti-tumor effects of SDT. MDR1 down-regulation via the Akt/NF-κB pathway by celecoxib was confirmed, using an NF-κB inhibitor, CAPÉ. Thus, elevation of cellar PpIX by down-regulation of MDR1 via the Akt/NF-κB pathway may be crucial to potentiate the efficacy of SDT in a site-directed manner and provide a promising new therapeutic strategy for GBM.
• DOI: info:doi/10.1038/s41598-021-93896-0; https://doi.org/info:doi/10.1038/s41598-021-93896-0
• Persistent ID (NDL): info:ndljp/pid/12356400; https://dl.ndl.go.jp/pid/12356400
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2022-10-11T11:55:44+09:00
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/116448
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp