Expression of N-terminal deficient E-cadherin protein in invasive lobular carcinoma of the breast

• Material Type: 博士論文
• Title: Expression of N-terminal deficient E-cadherin protein in invasive lobular carcinoma of the breast
• Author/Editor: 安井, 春奈
• Author Heading: 安井, 春奈
• Publication, Distribution, etc.: Wolters Kluwer Health, Inc.
• Publication Date: 2021-12-01
• Publication Date (W3CDTF): 2021-12-01
• Alternative Title: 乳腺浸潤性小葉癌におけるN末端欠損E-カドヘリン蛋白の発現
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2021-12-01
• Date Granted (W3CDTF): 2021-12-01
• Dissertation Number: 甲医歯薬第1375号
• Degree Type: 博士(医学)
• Text Language Code: eng
• Subject Heading: breast cancer; Invasive lobular carcinoma; E-cadherin; N-terminal; C-terminal
• Target Audience: 一般
• Note (General): Invasive lobular carcinoma (ILC) of the breast is characterized by the discohesive growth of tumor cells, which is mainly associated with the complete loss of E-cadherin (E-cad) expression. However, some aberrant expression patterns of E-cad protein that are inconsistent with their morphologies have been reported in ILC. We report herein ILC cases expressing a new type of abnormal E-cad protein that lacks the N-terminal domain, but conserves the C-terminal domain on the cell membrane. Immunohistochemical staining of 299 ILC cases using specific antibodies against the N-terminal or C-terminal region of E-cad revealed that 227 (76%) cases showed loss of the membranous expression of both terminuses (N−/C−) and 72 (24%) cases showed expression of only the C-terminus (N−/C+). In all cases, the expression of p120-catenin and β-catenin coincided with the expression of the C-terminus of E-cad. Clinicopathologic analysis revealed that N−/C+ expression in ILC cells was significantly associated with the histologic subtype (especially mixed-type ILC with another histologic type) and immunohistochemical molecular subtype (especially the triple-negative subtype), but not with prognostic factors (pT or pN). In addition, 12 of 15 cases (80%) with aberrant cytoplasmic localization of the N-terminal of E-cad showed diffuse membranous expression of the C-terminal domain. Additional immunohistochemistry using an antibody recognizing the extracellular juxtamembrane region showed that 28 (39%) of the N−/C+ cases had lost membranous expression, suggesting diversity in the deletion pattern of the N-terminal region. Our findings provide a novel mechanism for the loss of E-cad function because of N-terminal-deficient E-cad protein in ILC.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1375号 学位授与年月日:令和3年12月1日; Author: Haruna Yasui, Takuya Kawata, Koji Muramatsu, Yuko Kakuda, Takuma Oishi, Tomoko Norose, Akifumi Notsu, Seiichiro Nishimura, Junya Fukuoka, Takashi Sugino; Citation: The American Journal of Surgical Pathology, doi: 10.1097/PAS.0000000000001822; 2021; identifier:Nagasaki University (長崎大学), 博士(医学) (2021-12-01); http://hdl.handle.net/10069/00041136
• Persistent ID (NDL): info:ndljp/pid/12651160; https://dl.ndl.go.jp/pid/12651160
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• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2023-03-02T11:18:09+09:00
• Format (IMT): application/pdf
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• Periodical Title (URI): http://hdl.handle.net/10069/00041262
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