Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes.

• Material Type: 博士論文
• Title: Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes.
• Author/Editor: Ishikawa, Shoko; Nikaido, Misaki; Otani, Takahito; Ogata, Kayoko; Iida, Hiroshi; Inai, Yuko; Tamaoki, Sachio; Inai, Tetsuichiro
• Author Heading: Ishikawa, Shoko; Nikaido, Misaki; Otani, Takahito; Ogata, Kayoko; Iida, Hiroshi; Inai, Yuko; Tamaoki, Sachio; Inai, Tetsuichiro
• Publication Date: 2022-03-31
• Publication Date (W3CDTF): 2022-03-31
• Alternative Title: Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes.
• Degree grantor/type: 福岡歯科大学
• Date Granted: 2022-03-31
• Date Granted (W3CDTF): 2022-03-31
• Dissertation Number: 甲第339号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: Retinoic acid; Retinoid X receptor; Keratinocyte; Three-dimensional culture; Desmosome; Tight junction
• Target Audience: 一般
• Note (General): Retinoic acid (RA) plays an important role in epithelial homeostasis and influences the morphology, proliferation, differentiation and permeability of epithelial cells. Mouse keratinocytes, K38, reconstituted non-keratinized stratified epithelium in three-dimensional (3D) cultures with serum, which contains retinol (a source of RA), but the morphology was different from in vivo epithelium. The formed epithelium was thick, with loosened cell-cell contacts. Here, we investigated whether the inhibition of RA receptor (RAR)/retinoid X receptor (RXR)-mediated signaling by an RXR antagonist, HX 531, improved K38 3D cultures in terms of morphology and intercellular junctions. The epithelium formed by 0.5 μM HX531 was thin, and the intercellular space was narrowed because of the restoration of the layer-specific distribution of desmoglein (DSG)-1, DSG3 and plakoglobin (PG). Moreover, the levels of desmosomal proteins and tight junction proteins, including DSG1, DSG2, DSG3, PG, claudin (CLDN)-1 and CLDN4 increased, but the adherens junction protein, E-cadherin, did not show any change. Furthermore, CLDN1 was recruited to occludin-positive cell-cell contacts in the superficial cells and transepithelial electrical resistance was increased. Therefore, K38 3D cultures treated with 0.5 μM HX531 provides a useful in vitro model to study intercellular junctions in the non-keratinized epithelium.; OXFORD
• Persistent ID (NDL): info:ndljp/pid/12701610; https://dl.ndl.go.jp/pid/12701610
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2023-03-09T09:37:53+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): https://fdc.repo.nii.ac.jp/records/148
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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