Resveratrol inhibits development of colorectal adenoma via suppression of LEF1; comprehensive analysis with connectivity map

• Material Type: 博士論文
• Title: Resveratrol inhibits development of colorectal adenoma via suppression of LEF1; comprehensive analysis with connectivity map
• Author/Editor: Wada, Hironori; Sato, Yasushi; Fujimoto, Shota; Okamoto, Koichi; Bando, Masahiro; Kawaguchi, Tomoyuki; Miyamoto, Hiroshi; Muguruma, Naoki; Horimoto, Katsuhisa; Matsuzawa, Yui; Mutoh, Michihiro; Takayama, Tetsuji
• Author Heading: Wada, Hironori; Sato, Yasushi; Fujimoto, Shota; Okamoto, Koichi; Bando, Masahiro; Kawaguchi, Tomoyuki; Miyamoto, Hiroshi; Muguruma, Naoki; Horimoto, Katsuhisa; Matsuzawa, Yui; Mutoh, Michihiro; Takayama, Tetsuji
• Publication, Distribution, etc.: Japanese Cancer Association|John Wiley & Sons
• Publication Date: 2022-09-09
• Publication Date (W3CDTF): 2022-09-09
• Alternative Title: ResveratrolはLEF1を抑制することで大腸腺腫の発生を抑制する；connectivity mapを用いた網羅的解析
• Degree grantor/type: 徳島大学
• Date Granted: 2023-01-26
• Date Granted (W3CDTF): 2023-01-26
• Dissertation Number: 甲第3670号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: chemoprevention; colorectal adenoma; connectivity map; LEF1; resveratrol
• Target Audience: 一般
• Note (General): Although many chemopreventive studies on colorectal tumors have been reported, no effective and safe preventive agent is currently available. We searched for candidate preventive compounds against colorectal tumor comprehensively from United States Food and Drug Administration (FDA)-approved compounds by using connectivity map (CMAP) analysis coupled with in vitro screening with colorectal adenoma (CRA) patient-derived organoids (PDOs). We generated CRA-specific gene signatures based on the DNA microarray analysis of CRA and normal epithelial specimens, applied them to CMAP analysis with 1309 FDA-approved compounds, and identified 121 candidate compounds that should cancel the gene signatures. We narrowed them down to 15 compounds, and evaluated their inhibitory effects on the growth of CRA-PDOs in vitro. We finally identified resveratrol, one of the polyphenolic phytochemicals, as a compound showing the strongest inhibitory effect on the growth of CRA-PDOs compared with normal epithelial PDOs. When resveratrol was administered to ApcMin/+ mice at 15 or 30 mg/kg, the number of polyps (adenomas) was significantly reduced in both groups compared with control mice. Similarly, the number of polyps (adenomas) was significantly reduced in azoxymethane-injected rats treated with 10 or 100 mg/resveratrol compared with control rats. Microarray analysis of adenomas from resveratrol-treated rats revealed the highest change (downregulation) in expression of LEF1, a key molecule in the Wnt signaling pathway. Treatment with resveratrol significantly downregulated the Wnt-target gene (MYC) in CRA-PDOs. Our data demonstrated that resveratrol can be the most effective compound for chemoprevention of colorectal tumors, the efficacy of which is mediated through suppression of LEF1 expression in the Wnt signaling pathway.
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• Date Accepted (W3CDTF): 2023-05-05T22:12:32+09:00
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• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/117988
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