Porphyromonas gingivalis Outer Membrane Vesicles Stimulate Gingival Epithelial Cells to Induce Pro-Inflammatory Cytokines via the MAPK and STING Pathways

• Material Type: 博士論文
• Title: Porphyromonas gingivalis Outer Membrane Vesicles Stimulate Gingival Epithelial Cells to Induce Pro-Inflammatory Cytokines via the MAPK and STING Pathways
• Author/Editor: Uemura, Yuta; Hiroshima, Yuka; Tada, Ayano; Murakami, Keiji; Yoshida, Kaya; Inagaki, Yuji; Kuwahara, Tomomi; Murakami, Akikazu; Fujii, Hideki; Yumoto, Hiromichi
• Author Heading: Uemura, Yuta; Hiroshima, Yuka; Tada, Ayano; Murakami, Keiji; Yoshida, Kaya; Inagaki, Yuji; Kuwahara, Tomomi; Murakami, Akikazu; Fujii, Hideki; Yumoto, Hiromichi
• Publication, Distribution, etc.: MDPI
• Publication Date: 2022-10-20
• Publication Date (W3CDTF): 2022-10-20
• Alternative Title: Porphyromonas gingivalisの外膜小胞は歯肉上皮細胞を刺激しMAPKとSTING経路を介して炎症性サイトカインを産生させる
• Degree grantor/type: 徳島大学
• Date Granted: 2023-03-23
• Date Granted (W3CDTF): 2023-03-23
• Dissertation Number: 甲第3712号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: Porphyromonas gingivalis; outer membrane vesicles; stimulator of interferon genes; inflammation; periodontitis
• Target Audience: 一般
• Note (General): Porphyromonas gingivalis (Pg) is a keystone pathogen associated with chronic periodontitis and produces outer membrane vesicles (OMVs) that contain lipopolysaccharide (LPS), gingipains, and pathogen-derived DNA and RNA. Pg-OMVs are involved in the pathogenesis of periodontitis. Pg-OMV-activated pathways that induce the production of the pro-inflammatory cytokines, interleukin (IL)-6, and IL-8 in the human gingival epithelial cell line, OBA-9, were investigated. The role of mitogen-activated protein kinase (MAPK) and nuclear factor (NF)-κB in levels of Pg-OMV-induced pro-inflammatory cytokines was investigated using Western blot analysis and specific pathway inhibitors. Pg-OMVs induced IL-6 and IL-8 production via the extracellular signal-regulated kinase (Erk) 1/2, c-Jun N-terminal kinase (JNK), p38 MAPK, and NF-κB signaling pathways in OBA-9 cells. In addition, the stimulator of interferon genes (STING), an essential innate immune signaling molecule, was triggered by a cytosolic pathogen DNA. Pg-OMV-induced IL-6 and IL-8 mRNA expression and production were significantly suppressed by STING-specific small interfering RNA. Taken together, these results demonstrated that Pg-OMV-activated Erk1/2, JNK, p38 MAPK, STING, and NF-κB signaling pathways resulting in increased IL-6 and IL-8 expression in human gingival epithelial cells. These results suggest that Pg-OMVs may play important roles in periodontitis exacerbation by stimulating various pathways.
• Persistent ID (NDL): info:ndljp/pid/12911096; https://dl.ndl.go.jp/pid/12911096
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2023-07-08T03:42:29+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
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• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/117616
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp