miR-144-3p/miR-451a promotes lymphovascular invasion through repression of PTEN/p19 in rectal neuroendocrine tumors

• Material Type: 博士論文
• Title: miR-144-3p/miR-451a promotes lymphovascular invasion through repression of PTEN/p19 in rectal neuroendocrine tumors
• Author/Editor: Murayama, Noriaki; Okamoto, Koichi; Nakagawa, Tadahiko; Miyoshi, Jinsei; Nishida, Kensei; Kawaguchi, Tomoyuki; Kagemoto, Kaizo; Kitamura, Shinji; Ma, Beibei; Miyamoto, Hiroshi; Muguruma, Naoki; Yano, Mitsuyasu; Tsuneyama, Koichi; Fujimori, Takahiro; Sato, Yasushi; Takayama, Tetsuji
• Author Heading: Murayama, Noriaki; Okamoto, Koichi; Nakagawa, Tadahiko; Miyoshi, Jinsei; Nishida, Kensei; Kawaguchi, Tomoyuki; Kagemoto, Kaizo; Kitamura, Shinji; Ma, Beibei; Miyamoto, Hiroshi; Muguruma, Naoki; Yano, Mitsuyasu; Tsuneyama, Koichi; Fujimori, Takahiro; Sato, Yasushi; Takayama, Tetsuji
• Publication, Distribution, etc.: Journal of Gastroenterology and Hepatology Foundation|Wiley
• Publication Date: 2022-03-24
• Publication Date (W3CDTF): 2022-03-24
• Alternative Title: 直腸神経内分泌腫瘍において、miR-144-3p/miR-451aはPTEN/p19を介して脈管侵襲を促進する
• Degree grantor/type: 徳島大学
• Date Granted: 2022-10-27
• Date Granted (W3CDTF): 2022-10-27
• Dissertation Number: 甲第3664号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: 直腸NET; miR-144-3p; miR-451a; PTEN; p19; rectal NET-G1; lymphovascular invasion; miR-144-3p/451a
• Target Audience: 一般
• Note (General): Background and Aim: Although rectal neuroendocrine tumor (NET-G1) have potential metastatic capability, even among small tumors, no predictive biomarker for invasion and metastasis has been reported. We analyzed microRNA (miRNA) expression profiles in rectal NET-G1 tissues with and without lymphovascular invasion (LVI). Moreover, we then investigated their target genes to clarify the mechanism of invasion/metastasis in NET-G1.Methods: miRNA array analysis was performed using 7 rectal NET-G1 tissues with LVI and 7 without LVI. miRNA expression was confirmed by quantitative real-time PCR. A NET cell line H727 was transfected with miRNA mimic or target gene small interfering RNA, and migration and invasion assays were performed.Results: The expression levels of miR-144-3p and miR-451a were significantly higher in NET-G1 with LVI versus without LVI, as determined by miRNA array analysis and RT-qPCR. A significant correlation was observed between miR-144-3p and miR-451a expression levels, strongly suggesting miR144/451 cluster overexpression in NET-G1 with LVI. Bioinformatic analysis of target genes revealed that miR-144-3p and miR-451a directly interact with PTEN and p19 mRNA, respectively. Immunohistochemistry revealed significantly lower expression of PTEN and p19 in NET-G1 tissues with LVI than in those without LVI. The miR-144-3p and miR-451a mimic significantly increased cell migration/invasion capability, respectively. Knockdown of PTEN and p19 induced significant augmentation of cell invasion and migration capability, respectively.Conclusions: Our data suggest that overexpression of miR-144/miR-451 cluster promotes LVI via repression of PTEN and p19 in rectal NET-G1 cells. miR-144/451 cluster may be a novel biomarker for predicting invasion/metastasis in rectal NET-G1.
• Persistent ID (NDL): info:ndljp/pid/12911135; https://dl.ndl.go.jp/pid/12911135
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2023-07-08T03:42:30+09:00
• Format (IMT): application/pdf
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• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/117699
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