pruR and PA0065 Genes Are Responsible for Decreasing Antibiotic Tolerance by Autoinducer Analog-1 (AIA-1) in Pseudomonas aeruginosa

• Material Type: 博士論文
• Title: pruR and PA0065 Genes Are Responsible for Decreasing Antibiotic Tolerance by Autoinducer Analog-1 (AIA-1) in Pseudomonas aeruginosa
• Author/Editor: Pahlevi, Muhammad Reza; Murakami, Keiji; Hiroshima, Yuka; Murakami, Akikazu; Fujii, Hideki
• Author Heading: Pahlevi, Muhammad Reza; Murakami, Keiji; Hiroshima, Yuka; Murakami, Akikazu; Fujii, Hideki
• Publication, Distribution, etc.: MDPI
• Publication Date: 2022-06-06
• Publication Date (W3CDTF): 2022-06-06
• Alternative Title: pruRおよびPA0065遺伝子はAutoinducer Analog-1（AIA-1）による緑膿菌の抗菌薬耐性の低減に関与する
• Degree grantor/type: 徳島大学
• Date Granted: 2023-03-23
• Date Granted (W3CDTF): 2023-03-23
• Dissertation Number: 甲第3709号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: antibiotic tolerance; Pseudomonas aeruginosa; autoinducer analog-1; pruR; PA0066-65-64
• Target Audience: 一般
• Note (General): Pseudomonas aeruginosa infection is considered a high-risk nosocomial infection and is very difficult to eradicate because of its tolerance to antibiotic treatment. A new compound, autoinducer analog-1 (AIA-1), has been demonstrated to reduce antibiotic tolerance, but its mechanisms remain unknown. This study aimed to investigate the mechanisms of AIA-1 in the antibiotic tolerance of P. aeruginosa. A transposon mutant library was constructed using miniTn5pro, and screening was performed to isolate high tolerant mutants upon exposure to biapenem and AIA-1. We constructed a deletion mutant and complementation strain of the genes detected in transposon insertion site determination, pruR and PA0066-65-64, and performed killing assays with antibiotics and AIA-1. Gene expression upon exposure to biapenem and AIA-1 and their relationship to stress response genes were analyzed. High antibiotic tolerance was observed in Tn5-pruR and Tn5-PA0065 transposon mutants and their deletion mutants, ΔpruR and ΔPA0066-65-64. Complemented strains of pruR and PA0066-65-64 with their respective deletion mutants exhibited suppressed antibiotic tolerance. It was determined that deletion of PA0066-65-64 increased rpoS expression, and PA0066-65-64 affects antibiotic tolerance via the rpoS pathway. Additionally, antibiotics and AIA-1 were found to inhibit pruR and PA0066-65-64. This study proposed that pruR and PA0066-65-64 are members of the antibiotic tolerance suppressors.
• Persistent ID (NDL): info:ndljp/pid/12911211; https://dl.ndl.go.jp/pid/12911211
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2023-07-08T03:42:30+09:00
• Format (IMT): application/pdf
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• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/117279
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp