Assessment of insulin resistance in the skeletal muscle of mice using positron emission tomography/computed tomography imaging

• Material Type: 博士論文
• Title: Assessment of insulin resistance in the skeletal muscle of mice using positron emission tomography/computed tomography imaging
• Author/Editor: Miyatake, Yumiko; Mishima, Yuna; Tsutsumi, Rie; Otani, Tamaki; Suemasa, Naoya; Masumoto, Saeko; Kuroda, Masashi; Sakaue, Hiroshi
• Author Heading: Miyatake, Yumiko; Mishima, Yuna; Tsutsumi, Rie; Otani, Tamaki; Suemasa, Naoya; Masumoto, Saeko; Kuroda, Masashi; Sakaue, Hiroshi
• Publication, Distribution, etc.: Elsevier
• Publication Date: 2020-06-05
• Publication Date (W3CDTF): 2020-06-05
• Alternative Title: PET/CTを用いたマウス骨格筋インスリン抵抗性の評価
• Degree grantor/type: 徳島大学
• Date Granted: 2023-03-23
• Date Granted (W3CDTF): 2023-03-23
• Dissertation Number: 甲第3706号
• Degree Type: 博士（栄養学）
• Text Language Code: eng
• Subject Heading: PET/CT; Insulin resistance; Skeletal muscle; Glucose metabolism; Diabetes; FDG
• Target Audience: 一般
• Note (General): Measuring glucose uptake in the skeletal muscle in vivo is an effective method to determine glucose metabolism abnormalities as the skeletal muscle is the principal tissue responsible for glucose disposal and is a major site of peripheral insulin resistance. In this study, we investigated the pathological glucose metabolism dynamics of the skeletal muscle of C57BL/6J mice in a noninvasive and time-sequential manner using positron emission tomography/computed tomography (PET/CT), an imaging technique that uses radioactive substances to visualize and measure metabolic processes in the body, with [18F]-fluoro-2-deoxy-D-glucose (FDG). FDG-PET/CT imaging revealed that insulin administration and exercise load significantly increased FDG accumulation in the skeletal muscle of C57BL/6J mice. FDG accumulation was lower in the skeletal muscle of 14-week-old db/db diabetic model mice exhibiting remarkable insulin resistance compared to that of 7-week-old db/db mice. Based on the continuous observation of FDG accumulation over time in diet-induced obese (DIO) mice, FDG accumulation significantly decreased in 17-week-old mice after the acquisition of insulin resistance. Although insulin-induced glucose uptake in the skeletal muscle was markedly attenuated in 20-week-old DIO mice that had already developed insulin resistance, exercise load effectively increased FDG uptake in the skeletal muscle. Thus, we successfully confirmed that glucose uptake accompanied by insulin administration and exercise load increased in the skeletal muscle using PET-CT. FDG-PET/CT might be an effective tool that could noninvasively capture the chronological changes of metabolic abnormalities in the skeletal muscle of mice.
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• Date Accepted (W3CDTF): 2023-07-08T03:42:30+09:00
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• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/118304
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